乙型肝炎核心抗体阳性与阴性慢性乙型肝炎患者血清可溶性白介素2受体检测

Detection of serum soluble interleukin-2 receptor in anti-hepatitis B core positive and negative patients with chronic hepatitis B

目的 分析比较慢性乙型肝炎核心抗体 (抗 HBc)阳性的患者和抗 HBc阴性的患者血清中可溶性白细胞介素 2受体 (sIL 2R)和乙型肝炎病毒DNA(HBV DNA)含量的变化及意义。方法 将 10 0例诊断为慢性乙型肝炎肝者分为两组 ,A组为 5 0例 ,HBsAg阳性、HBeAg阳性和抗 HBc阳性 ,B组为 5 0例 ,HBsAg阳性 ,HBeAg阳性和抗 HBc阴性。另选 30例健康体检者 ,HBV标志均为阴性 ,作为对照组。sIL 2R采用双抗体夹心间接酶联免疫吸附测定法 ,HBV DNA采用荧光定量聚合酶链法 ,丙氨酸转氨酶 (ALT)采用赖氏法。结果 乙型肝炎表面抗原 (HBsAg)阳性、乙型肝炎e抗原 (HBeAg)阳性、抗 HBc阳性组血清中sIL 2R和ALT水平均明显高于HBsAg阳性、HBeAg阳性、抗 HBc阴性组 ,P <0 .0 5 ,与正常对照组比较 ,P <0 .0 5 ,且年龄偏向于青少年段 ,但比较HBV DNA含量在两组患者差异无统计学意义 (P >0 .0 5 ) ,HBsAg阳性、HBeAg阳性、抗 HBc阴性组sIL 2R水平亦高于正常对照组 P <0 .0 5。结论 以上结果表明抗 HBc阴性的慢性乙型肝炎患者机体的免疫系统处于一种免疫耐受的状态 ,而sIL

S: Objective To investigate the role of serum soluble interleukin-2 receptor(sIL-2R) in patients with hepatitis B core antibody(anti-HBc) positive (HBcAb+) and patients with anti-HBc negative(HbcAb-),and change of hepatitis B virus DNA(HBV-DNA).Methods Chronic hepatitis B patients of 100 cases that were diagnosed were divided into two groups.Group A had 50 cases with HBsAg(+),HBeAg(+) and anti-HBc(+),Group B had 50 cases with HBsAg(+),HBeAg(+) and anti-HBc(-).Another 30 healthy cases taken from body check were served as control group with all HBV markers negative.Double antibody enzyme-linked immunosorbent assay and fluorogenic quantitative polymerase chain reaction were used to detect the level of sIL-2R and HBV-DNA respectively.Alanine aminotransferase(ALT) was detected with Reitman method.Results The levels of serum sIL-2R and ALT in the group with HBsAg+HBeAg+anti-HBc+ were significantly higher than those in the group with HBsAg+HBeAg+anti-HBc- and control group (P< 0.05),aging trend to adolescent.But HBV-DNA concentration was not found to have significant difference between the two patients′ groups.Conclusion Chronic hepatitis B patients with HBsAg+HBeAg+anti-HBc- showed the status of immunotolerance,sIL-2R can be served as an indicator for the status of the body immunity.