HPLC法测定羟甲烟胺的含量

Determination of Hydroxymethylnicotinamide with HPLC

目的 采用高效液相色谱法测定羟甲烟胺的含量。方法 以C18化学键合硅胶柱分离羟甲烟胺,以甲醇 -磷酸二氢钠(pH =6.0 )(10∶90 )为流动相,UV检测波长 2 6 1nm进行测定。结果 平均回收率为 99 76 %,RSD为 0 2 9%(n =5),羟甲烟胺进样量与吸收峰面积呈良好的线性关系。线性范围 0 1～ 0 6mg·ml-1。结论 该方法测定羟甲烟胺的含量,结果准确,重现性好。

Objective The content of Hydroxymethylnicotinamide was determined by HPLC.Methods Hydroxymethylnicotinamide was separated by C 18-silica gel column with methonal-natrium phosphate dibasic solution(pH=6.0)(10∶90) as the eluent,UV determination wave-length was 261nm.Results Average recovery of the method was 99.76%,RSD=0.29%(n=5),the content of Hydroxymethylnicotinamide showed good linear relation with peak areas,linearity range was 0.1～0.6mg·ml -1. Conclusion This method was accurate and suitable for Hydroxymethylnicotinamide content determination.