瘢痕疙瘩和增生性瘢痕中胸腺素β4基因表达变化及其意

Expression of thymosin β4 mRNA expression in keloid tissues and fibroblasts cultured from keloid and its significance

目的 观察病理性瘢痕中胸腺素β4 (TMSB4 ) m RNA的表达水平 ,探讨其表达水平与病理性瘢痕形成的关系。方法 以组织块培养法于体外培养原代成纤维细胞。采用半定量逆转录聚合酶链反应(RT PCR)技术 ,比较瘢痕疙瘩、增生性瘢痕和正常皮肤组织及其培养的 3组成纤维细胞中 TMSB4 m RNA表达水平的变化。结果 在上述 3种组织中 ,TMSB4 m RNA在瘢痕疙瘩中的表达量显著少于在增生性瘢痕和正常皮肤中的表达 (P均 <0 .0 1) ,瘢痕疙瘩 TMSB4 m RNA表达的平均值比增生性瘢痕减少 6 6 .98% ,比正常皮肤减少 6 2 .4 8%。在上述 3种组织培养的成纤维细胞中 ,TMSB4 m RNA在瘢痕疙瘩成纤维细胞中的表达量显著少于增生性瘢痕 ,平均减少 2 7.13% (P<0 .0 1) ;比在正常皮肤成纤维细胞中的表达平均减少 16 .0 7% ,但差异无显著性。结论 TMSB4 m RNA在瘢痕疙瘩组织及其培养的成纤维细胞中的表达说明其与瘢痕疙瘩形成密切相关 ;TMSB4 m RNA在瘢痕疙瘩中表达减少可能是瘢痕疙瘩发病的重要致病因素之一。

Objective To investigate the relations between thymosin β4 and pathologic scars by comparing the mRNA levels of thymosin β4 in keloid, hypertrophic scar and normal skin. Methods The primary fibroblasts from the patients of keloid (KFB), hypertrophic scar (HFB) and normal skin (NFB, n =7 ) were cultured in vitro with tissue culture system. Semiquantitative reverse transcriptionpolymerase chain reaction (RTPCR) assay was used to assess the thymosin β4 mRNA levels in the tissues and fibroblasts obtained from the patients of three groups. Results The mRNA levels of thymosin β4 in the keloid tissues were lower than those in the tissues of hypertrophic scar and normal skin(both, P <0 01). The thymosin β4 mRNA level in keloid group was lower by 66 98% than hypertrophic scar, and 62 48% than normal skin. In addition, the same significant change was found in the cultured KFB compared with HFB group(the mean value was lower by 27 13%), but no difference was found between KFB and NFB. Conclusion Expression of thymosin β4 is closely related with keloid. The inadequate expression of thymosin β4 may be one of the key factors for keloid formation.