胰岛素抵抗大鼠血清NO、血管紧张素Ⅱ和超氧阴离子水平的变化

The Changes of Serum Nitric Oxide, Angiotensin II and Superoxide Anion in Insulin Resistance Rats

目的探讨胰岛素抵抗大鼠血清一氧化氮(NO)、血管紧张素Ⅱ(AngII)和超氧阴离子(O2-)水平的变化。方法应用高蔗糖饮食诱发大鼠胰岛素抵抗模型,实验分为对照组、高糖组、高糖+L鄄精氨酸组、高糖+开搏通组,分别测定大鼠血清NO、AngⅡ和O2-的变化。采用SPSS8.0统计软件处理。结果高糖组、高糖+L鄄精氨酸组大鼠血清NO高于对照组(P<0.01),高糖+开搏通组大鼠血清NO与对照组比较差别无统计学意义(P>0.05)。高糖组大鼠血浆cGMP水平较对照组降低(P<0.05),高糖+L鄄精氨酸组cGMP高于对照组,高糖+开搏通组与对照组比较,差别无统计学意义。高糖+L鄄精氨酸组和高糖+开搏通组cGMP水平高于高糖组(P均<0.01)。高糖组、高糖+L鄄精氨酸组大鼠血清O2-水平高于对照组(P<0.01),高糖+开搏通组大鼠血清O2-水平与对照组比较差别无统计学意义(P>0.05)。高糖组和高糖+L鄄精氨酸组大鼠血清AngⅡ水平高于对照组(P<0.01),高糖+开搏通组大鼠血清AngⅡ水平与对照组比较差别无统计学意义(P>0.05),但低于高糖组和高糖+L鄄精氨酸组(P<0.01)。结论胰岛素抵抗大鼠血清NO生物利用度下降,血清AngⅡ和O2-水平升高,L鄄精氨酸可以增加NO的生成,使cGMP升高,引起血压下降,开搏通通过阻断AngⅡ的生成,减少O2-的产生,增加NO生物利用度,使血压下降。L鄄精?

Objective To study the changes of serum nitric oxide(NO), angiotensin II(AngII) and superoxide anion(O2-) in insulin resistance rats. Methods Rat insulin resistance model was set up using cane sugar rich diet, L-arginine and captopril was given respectively, at the end of the esperiment serum was drawn to assay NO?AngⅡ and O2-. Results Sixteen weeks after experiment, although blood glucose did not change significantly, but blood pressure, body weight and serum insulin elevated in cane sugar rich diet rats, in spite of NO elevated in glucose rich diet rats, the level of plasma cGMP was lower, that is to say NO bioavailability was lower, AngⅡand O2- increased in cane sugar rich diet group. L-arginine supplementation may increase NO bioavailability, prevent blood pressure elevating, captopril administration can reduce blood pressure, decrease O2- level, increase NO bioavailability by blocking AngⅡ generation, the effects of captopril stronger than that of L-arginine. Conclusion Cane sugar rich diet may induce rat hyperinsulinemia and hypertension, NO bioavailability decreased, serum AngⅡ and O2- level elevated, L-arginine and captopril prevented the effects of cane sugar rich diet on blood pressure, NO bioavailatility, AngⅡand O2-.