血小板源性生长因子对体外培养内皮细胞碱性成纤维细胞生长因子水平的影响

Effect of Platele-derived Growth Factor on Bisic Fibroblast Growth Factor for the Cueture of Endothelial Cells

目的 :观察血小板源性生长因子 (PDGF)对于培养脐静脉内皮细胞碱性成纤维细胞生长因子 (bFGF)水平的影响 ,探讨PDGF促进新生血管形成的机制。方法 :通过内皮细胞的培养和免疫组织化学等方法检测PDGF对缺氧和常规培养内皮细胞bFGF水平的影响以及不同浓度PDGF促bFGF表达的作用。结果 :缺氧培养与常规培养均能增加内皮细胞胞浆bFGF水平 ,且呈剂量依赖性。结论 :PDGF可能通过直接促血管形成生长因子bFGF的介导作用间接促进新生血管的形成。

Objective:To investigate platelet-derived growth factor(PDGF) modulate the expression of basic Fibrolast Growth Factor(bFGF) in human umbilical endothelial cells and PDGF influence mechanism on angiogensis.Method:The level of bFGF of endothelial cell was detected with Immunhistochemical.Results:The results as followed,level of bFGF have changes w hen cell under normal or hypoxia.Conclusion:These manifest shows PDGF promoted angiogensis by i ncreasing bFGF leves of endothelial cells.