重组TFPI-2影响人卵巢癌细胞体外迁徙浸润能力的研究

Inference of Recombinant TFPI-2 on the Migration and Invasion of Human Ovarian Tumor Cell in Vitro

目的 :从功能角度研究重组组织因子途径抑制物 2 (TFPI 2 )对人卵巢癌细胞体外迁徙、浸润能力的影响。方法 :①迁徙实验 ,加不同浓度TFPI 2培养的A2 780细胞和不加TFPI 2的A2 780细胞 ,通过Boyden小室体外浸润转移模型 ,以迁徙到聚碳脂微孔滤膜 (PVPF)背面的每高倍镜视野中的平均细胞数作为评价人卵巢癌细胞迁徙能力强弱的指标。②浸润实验在PVPF膜铺上基底膜基质胶 (Matrigematrix)后 ,行迁徙实验。结果 :①迁徙实验中 ,将A2 780 TFPI 2不同浓度组与A2 780对照组细胞穿过PVPF膜的细胞数进行比较 ,经t检验 ,没有统计学意义 (P >0 .0 5)。②浸润实验中 ,将A2 780 TFPI 2不同浓度组与A2 780对照组的细胞穿过人工膜的细胞数进行比较 ,及TFPI 2不同浓度组间的细胞数进行比较 ,经t检验 ,均有非常显著差异 (P <0 .0 1)。结论 :重组TF PI 2对人卵巢癌细胞体外自身运动能力无影响 ,但可显著抑制人卵巢癌细胞的体外浸润能力 ,为卵巢癌的蛋白酶抑制剂治疗提供一可能的靶向依据。

Objective:To investigate the role of recombinan TFPI-2 in huma n ovarian tumor cell migration and invasion in vitro.Method:Boyden chamber was used to test the ability of ovarian t umor cells migration and invasion in vitro.The number of A2780 and A2780-TFPI -2 cells passing through polyvinypyrrolidone-free polycarbonate filter(PVPF) m embrance in Boyden chamber was counted as the basis assessing ovarian tumor cell migratory and invasive behaviors.Results:In invasion assay,the number of A2780-TFPI-2 groups traversed the Marrigel matrix-coated PVPF membrance is obviously decreased com pared with that of A2780 group (P<0.01),While in migration assay,no signif icant difference is observed beteen A2780-TFPI-2 groups and A2780 groud(P >0.05).Conclusion:The recombinant TFPI-2 inhibits the invasive abilit y of human ovarian tumor cells in vitro,but has no effect on the migration.This may provide a target basis for treating human ovarian tumor with TFPI-2 protei n therapy.