骨髓间充质干细胞移植对豚鼠皮肤深Ⅱ度烧伤的治疗作用

Effect of bone marrow mesenchymal stem on the deep partial thickness derma burn injury in guinea pigs

目的探讨骨髓间充质干细胞 (MSCs)治疗皮肤深Ⅱ度烧伤的机制。方法体外培养扩增的MSCs悬液 ,以 2× 10 6/ml(A组 )和 2× 10 7/ml(B组 )两种细胞密度移植给皮肤深Ⅱ度烧的受体鼠创面 ,观察创面愈合速度 ,用免疫组织化学检测因子Ⅷ的表达。细胞移植后的第 15、3 0、5 0天 ,通过PCR方法检测创面皮肤组织中移植供体鼠的Y染色体基因表达。结果A、B组治疗侧的创面愈合速度均快于对照侧 ,A、B组治疗侧间无显著性差异。因子Ⅷ检测示第 15、3 0天时 ,A、B两组的微血管密度均多于对照侧 ,A、B组治疗侧间无显著性差异。PCR检测结果表明 ,第 15、3 0、5 0天时在部分受体鼠恢复的创面上有供体鼠Y染色体基因表达。结论骨髓MSCs对皮肤深Ⅱ度烧伤具有明显的疗效。

ObjectiveTo explore the effect of bone marrow mesenchymal stem cells transplantation on the deep partial thickness derma burn injury in guinea pigs.MethodsSuspension cells of MSCs were cultured in vitro and transplanted into wounds of the female guinea pig of the deep partial thickness derma burn injuring model with the concentration of 2×10 6 cell/ml(group A),2×10 7cell/ ml(group B). The rate of wound healing was observed, factor Ⅷ was detected using immunohistochemistry. Y chromosome on the wound in guinea pig was detected using PCR 15,30,50 days after MSCs had been transplanted. ResultsThe rate of wound healing of groups A and B was better than that in the control group, but there was not significantly different between groups A and B. Microvessel density of wound in groups A and B was better than those in the control group on the 15th and 30th days. There was not significantly different between the group A and B. PCR results showed that Y chromosome gene was expressed on some recoveried wound of female guinea pig. ConclusionBone marrow mesenchymal stem cells transplantation is effective in deep partial thickness derma burn injury in guinea pigs. [