小鼠黑色素瘤B16细胞空间无源搭载条件的筛选

Screening of conditions carrying murine melanoma B16 cells in spaceflights without care

目的筛选小鼠黑色素瘤B16细胞无源搭载的最佳条件。方法将小鼠黑色素瘤B16细胞纯化后接种在微载体上 ,按不同细胞浓度、血清浓度、微载体量和温度分组。 3 3d后 ,Giemsa染色 ,光境下观察 ,计算细胞存活率。结果细胞存活率 8%和10 %的两组为最佳条件。利用所选条件 ,将细胞搭载于第 2 0颗返回式卫星 ,空间飞行 18d ,细胞返回后 ,经单克隆化 ,获得 110株单克隆细胞 ,存活率为 1 1%。结论筛选出B16细胞无源搭载的最佳条件 ,实际空间飞行表明细胞在空间中的存活时间和生存率满意。

ObjectiveTo screen the optimum conditions carrying murine melanoma B16 cells in spaceflights without cares.MethodsMurine melanoma cells were cultured on micocarriers and grouped depending on cells concentration, serum concentration, microcarrier number and temperature. After 33 days, B16 cells were stained by Giemsa, observed with phase contrast microscope and counted for surviving percentage.ResultsThe optimum conditions, in which the surviving percentages were 8% and 10%, were obtained in the experiments. B16 cells were carried in the 20th recoverable satellite orbiting 18 days under the optimum conditions. After recovering, 110 strain monocloned cells were survived and the surviving percentage was 1 1%.ConclusionThe optimum conditions carrying murine melanoma B16 cells in spaceflights without cares seems to be obtained, and it did improve the surviving time and percentage of cells in spaceflights without cares. [