高胚胎再生能力大豆品种的筛选和培养方法改进(英文)

Screening of high somatic embryogenic soybean varieties and innovation of culture methods

应用离体培养的方法,从本地10个,巴西5个和美国2个合计17个大豆品种中筛选出廉江、浙春、Perry、BRS07和Chief等5个体细胞胚胎再生能力强的品种,并在此基础上对这5个品种的培养基生长素浓度进行优化.对于廉江、浙春、Perry和BRS,最合适的2,4-D质量浓度为2mg/L,而对于Chief的最合适质量浓度为5mg/L.此外,在以Perry和BRS07为材料进行的另一个试验中发现,当同为5mg/L的硫酸铜和硝酸银同时添加到培养基时对促进体细胞胚胎的形成具有协同作用.

Seventeen soybean (Glycine max L.)varieties representing 10 local, 5 Brazilian and 2 American ones were screened for their somatic embryogenic capacity using uniform in vitro culture methods. Auxin concentrations were optimized with five highest responsive varieties. The optimal concentration of 2,4-D for the varieties of Lianjiang, Zhechun 2, Perry and BRS 07 were found to be 2 mg/L, whereas that for Chief was 5 mg/L. Moreover, synergistic enhancing effects of two additives, copper sulfate and silver nitrate on embryogenesis were detected in separated experiments with Perry and BRS 07 when the media were supplemented with both at 5 mg/L.