肿瘤患者深部念珠菌感染的血清学诊断

Study on serological diagnosis of fungus infection of patients suffering from tumor

目的 探讨早期诊断肿瘤患者念珠菌感染的血清学检测方法。方法 用CAND TEC念珠菌检测试剂和sandfidiagnosticspasteur丝状真菌抗原检测试剂检测 15 4例真菌培养阳性肿瘤患者血清 ,同期选择临床及微生物学检查均排除念珠菌感染的住院肿瘤患者 2 1例和健康志愿者 9名作为血清念珠菌抗原检测的阴性对照。结果 880例肿瘤患者中 15 4例念珠菌培养阳性 ,经临床观察确定 5 2例为念珠菌感染者 ,经血清念珠菌抗原检查有 4 8例阳性 ,阳性率为 92 3% ;而对照组 30例中仅有 1例阳性 ,阳性率 3 3% (χ2 =5 8 98,P <0 0 1)。结论 单纯依靠念珠菌培养是否阳性不足以鉴别感染者或带菌者 ,临床符合率仅 33 8% ,而用念珠菌抗原检测 ,与临床感染症状一致率达 92 3% ,是快速、准确地诊断肿瘤患者深部念珠菌感染的有效方法之一。

Objective To establish a serological detecting method for early diagnosis fungus infection of patients suffering from tumor.Methods 154 of 880 cases of tumor patients were examined by the antigen detecting reagents of CAND TEC for candida and Sanofi Diagnostics Pasteur for filamentous form fungus Simultaneously also chosen 21 cases of tumor inpatients who were free of fungus infection confirmed by clinical examined and microbiological detect and 9 cases of normal donor were chosen as the negative control Results 154 of 880 cases were confirmed whom with fungus infection depend on clinical observed and fungus antigen detecting 48 of 52 cases were positive of serological fungus antigen detecting.The positive rate was 92 3%.In 30 cases of control group,there was only one cases positiveTthe positive rate was 3 3%( χ 2=58 98, P <0 01) Conclusions Only the fungus culture results were not enough to confirm the infection of patients,as the identical rate with clinical aspect was only 33 8%.While the positive rate of serological fungus antigen detecting methods was 92 3%.In one hour,we can get the testing result Therefore,the serological fungus antigen test was the significant rapid,in time method to confirm with innerdepth fungus infection