丹参对实验性动脉粥样硬化形成的预防

Effect of danshen on experimental atherosclerosis

目的:探讨丹参抑制动脉粥样硬化(AS)作用中的可能的分子机制。方法:随机将21只家兔均分为 对照组、AS组和丹参干预组,采用高脂饲料建立AS模型,9周后行血脂测定、主动脉粥样斑块面积计算,并以逆 转录 聚合酶链式反应(RT PCR)分别进行三组的单核细胞趋化蛋白 1(MCP 1)mRNA表达水平测定,以及用免 疫组织化学方法对比各组MCP 1蛋白表达。结果:对照组和丹参组中血脂水平和主动脉粥样斑块面积低于AS 组(P<0.01);主动脉血管内皮细胞以及平滑肌细胞内MCP 1mRNA水平及蛋白表达水平在对照组和丹参组中 明显低于AS组(P<0.01)。结论:丹参能降低MCP 1表达水平,这可能是其抑制AS形成的分子学机制之一。

Objective:To explore the molecular mechanism of danshen in experimental atherosclerosis(AS). Method:Twenty-one normal male rabbits were randomly divided into three groups: normal control group, AS group and danshen treating group. Hyperlipidic feed was adopted to build the atherosclerotic animal model. Meanwhile, we used Danshen to intervene atherosclerosis in the animal model. Serum lipids and aorta tissues were measured after nine weeks.The effect of Danshen on the level of Monocyte chemotatic protein-1 (MCP-1) mRNA expression and MCP-1 protein were observed and analyzed by means of reverse transcription-polymerase chain reaction assay (RT-PCR) and immunohistochemical method. Result: Serum TG and the aorta atherosclerosis area in normal group and danshen group were significantly lower than that in AS group (P< 0.01), MCP-1 expression was reduced in danshen group (P< 0.01). Conclusion:Danshen is able to reduce expression level of MCP-1, which is one of the possible molecular mechanisms in inhibiting atherosclerosis.