左归双降方对葡萄糖胰岛素低密度脂蛋白诱导损伤的血管内皮细胞ICAM-1tPA PAI的影响

The Effects of Zuo Gui Shuang Jiang Fang on ICAM-1,tPA,and PAI of the Vascular Endothelial Cells Injured by Glucose,Insulin and Low-Density Lipoprotein

目的 :观察左归双降方提取液对葡萄糖 (Glu)、胰岛素 (Ins)、氧化低密度脂蛋白 (ox -LDL)联合诱导下血管内皮细胞损伤的干预作用与作用机理。方法 :以人脐带静脉内皮细胞株ECV -3 0 4为受试对象 ,以不同浓度的Glu、Ins、ox -LDL联合作用 2 4h ,建立了一种糖尿病血管并发症的体外内皮细胞损伤模型 ;并在此基础上观测左归双降方提取液 (ZGSJF)对该细胞损伤模型的调节作用。各试验组细胞继续培养 48h后分别检测细胞活性及其细胞培养液中的细胞黏附因子 (ICAM -1)、组织型纤溶酶原激活物 (tPA)、纤溶酶原激活物抑制物 (PAI)的含量。结果 :细胞继续培养 48h后 ,模型组较正常组细胞活力明显下降 (P <0 0 1) ,PAI及ICAM -1含量明显升高 (P <0 0 1)。而不同剂量的ZGSJF给药后能显著地改善细胞活力 (与模型组比较P <0 0 5 ) ,降低PAI及ICAM -1含量 (与模型组比较P <0 0 1) ,对tPA的影响则无显著性意义。结论 :左归双降方对血管内皮细胞具有一定的保护作用 ,而这一作用可能是通过促进内皮细胞的纤溶功能 ,抑制与炎症反应相关的细胞粘附过程等途径 ,从而改善糖尿病血管病变的血管损伤。

Objective :To investigate the effects of Zuoguishuangjiangfang(ZGSJF) on vascular endothelial cells injured by glucose,insulin and low density lipoprotein. Methods :We made human umbilical vein endothelial cell line ECV 304 exposed to different concentration of glucose,insulin and oxidized low density lipoprotein(Ox LDL)for 24 hours,and to investigate the effects of ZGSJF on this injured vascular endothelial cells.The cells in each group were cultured for additional 48 hours.And then,cell viability was examined,and the supernatants were used to determine the contents of tissue plasminogen activator (tPA),plasminogen activator inhibitor(PAI),and intercellular adhesion molecule 1(ICAM 1)respectively. Results :After the cells in each group were cultured for additional 48 hours,we found that the viability(measured by MTT test)of the cells in model group decreased markedly( P <0 01 vs blank group),and the secretion of PAI,and ICAM 1 in the model group was significantly higher than that of the blank group( P <0 01).After treating of different dose ZGSJF,the cell viability was significantly greater than that of the model group( P <0 05);The cells′PAI and ICAM 1 were significantly lower than those of the model group( P <0 01). Conclusion :The ZGSJF had the determinate protective function to the vascular endothelial cells,and this function might be realized by advancing fibrinolysis and inhibiting intercellular adhesion in order to decrease diabetic vasculopathy.