幼龄小鼠短期甲基汞中毒致细胞凋亡

Experimental study on apoptosis of infant mice with short term MeHg poisoning

目的：探讨甲基汞所致免疫损伤机制。方法：将幼龄小鼠分为6组，除对照组外实验5组分别各皮下注射甲基汞1、2、4、8、10mg，(kg．d)，连续7d，15d后取胸腺，进行形态学、流式细胞术和DNA琼脂糖凝胶电泳等观测，研究不同剂量组小鼠胸腺细胞凋亡变化。结果：甲基汞诱导小鼠胸腺细胞凋亡，凋亡率随剂量增大而增加，第15天凋亡率从(5．42±0．95)％增至(59．30±1．17)％；光镜下实验3、4、5组均观察到胸腺细胞凋亡的典型形态变化；琼脂糖凝胶电泳结果呈“梯形”条带。结论：甲基汞可诱导小鼠胸腺细胞凋亡。

Objective:To approach immunologic injury mechanism resulted from MeHg.Methods:With infant mice divided into six groups,we injected MeHg into subcutaneous tissue of mice in 5 tested groups for 7 days on end.The doses were respectively 1,2,4,8,10 mg/kg every day.The thymus cells were taken out on the 15th day for the observation of morphology,flow cytometry and DNA electrphoresis,and then study the apoptosis changes of thymus cells in different dose groups.Results:MeHg led to thymus apoptosis in infant mice,...