成年大鼠骨髓基质细胞与划痕损伤星形胶质细胞相互作用后的形态学观察

Morphological changes after interaction between adult rat marrow stromal stem cells and scratched astrocytes

目的通过研究骨髓基质细胞(MSCs)与星形胶质细胞的相互作用,探索MSCs修复中枢神经系统损伤的机制。方法体外培养Wistar大鼠MSCs和Wistar乳鼠海马来源的星形胶质细胞,并将两种细胞共培养,然后在相差显微镜下观察其生长状态,扫描电镜下观察其超微结构变化,对照组为单一的MSCs与星形胶质细胞。结果共培养后第6天的MSCs、星形胶质细胞与对照组比较,存活状态良好;超微结构观察显示星形胶质细胞损伤明显减轻,可见明显的微绒毛、长突起、丝状伪足,其周围及表面聚集大量的MSCs,二者关系密切,部分MSCs呈典型的神经元样外观,并与星形胶质细胞有类突触样结构形成。结论①MSCs与星形胶质细胞能够在体外相互促进生长;②星形胶质细胞具有调控神经元分化的功能。

Objective To explore whether the adult rat marrow stromal stem cells (rMSCs) and astrocytes can promote growth each other in vitro during the repair after central nervous system injury. Methods Astrocytes were isolated from the hippocampus of newborn Wistar rats and rMSCs from the femurs and tibias of 8- to 12- week-old Wistar rats. The two kinds of cells were co-cultured, then observed under contrast and electron microscopes for the morphological changes, and compared with single astrocytes or single rMSCs. Results At the sixth day after co-culture, rMSCs and astrocytes grew well comparing with the control group. Ultrastructure revealed the astrocytes in co-culture group had obvious microvilli, long processes, and filopodia. rMSCs were closely related with astrocytes and some had typical appearance of neurons. Conclusions ①rMSCs and astrocytes can promote the growth each other in vitro. ②Astrocytes have an active regulatory role and promote the neuronal differentiation of rMSCs.