显色底物比色法测定人血浆中t-PA、PAI活性及其临床意义

Chromogenic Substrate Colorimetric Assay for Plasma t-PA and PAl and Their Clinical Significance

组织型纤溶酶原激活剂(t-PA)、纤溶酶原激活剂抑制剂(PAI)均是体内纤溶系统的重要组成部分。t-PA、PAI 活性测定对某些血栓性疾病的研究有重要的临床应用价值。本文采用显色底物比色法对人体血浆中 t-PA、PAI 活性进行了测定,结果表明本法灵敏度高,稳定性好。对照组 t-PA 活性为1.55±0.67 IU/ml,PAI 活性为8.77±3.07 AU/ml。心肌梗塞、脑梗塞和冠心病患者 t-PA 活性较正常水平下降,PAI 活性则明显升高。

PAI and t-PA are important constituents of fibrinolytic system in human body.Chromogenic substrate colorimetric estimation was used to detect t-PA and PAI inplasma of patients with myocardial infarctions,brain thrombosis and coronany disease.It was shown that decrease of t-PA and increase of PAI in different levels as compa-red with normal subjects.