用HPLC法研究不同提取方法对人参单体皂甙的提取效果

A COMPARISION OF GINSENOSIDES FROM PANAX GINSENG EXTRACTS USING DIFFERENT EXTRACTANTS BY HPLC METHOD

本文采用MPG-ODS色谱柱,以醋酸铵作HPLC流动相的改性荆在15 min以内较好地分离出单体皂甙Rg_2、Rb_1、Rc、Rd、Rg_1、Re等,首次比较了化学上醇提取方法和食用时水提取方法对单体皂甙及总皂甙提取效果的不同,结果表明,食用水对Rg组类皂甙的提取量高于Rb组,这将为人参的药理学研究及临床食用提供科学依据。

Six ginsenosides(Rg_2、Rb_1、Rc、Rd、Rg_1、Re) were separated by HPLC using a column of octadecylsilyl chemically modified porous glass(MPG—ODS) within 15 min. and eluted with acetonitrile-water-ammonium acetate with UV detection at 204nm. Two different methods of extracting ginsenosides from the crugs were compared by HPLC method for ginsenoside assay, one extd. wiht n-BuOH satrd. H_2O, another with edible water. Our experimental results showed that the extractability to the ginsenoside group of Rg is higher than to the ginsenoside group of Rb with edible water, which would provide scientific basis for the pharmacology study on Panax Ginseng.