慢性萎缩性胃炎细胞DNA倍性、增殖活性检测的意义

Significance of Detecting DNA Ploidy and Proliferation Activity in Chronic Atrophic Gastritis

用流式细胞术(FCM)检测59例慢性萎缩性胃炎(CAG)、74例胃癌细胞 DNA倍性、S期细胞比率(SPF)和增殖指数(PI),同时以18例正常胃粘膜作为对照。结果:单纯 CAG4.35%检出异倍体,CAG 伴肠化生和不典型增生及胃癌的异倍体检出率分别为25%和66.23%,差异有显著性(P<0.05)。从对照组到单纯 CAG、CAG 伴肠化生不典型增生至胃癌,SPF 和 PI递增,各组之间有显著差异(P<0.05或 P<0.01)。FCM 细胞 DNA 倍性、SPF 和 PI 分析有助于胃癌癌前病变监测。

DNA ploidy,S-phase fraction(SPF)and proliferation index(PI)were detectedby flow cytometry(FCM)in 59 cases of chronic atrophic gastritis(CAG)and 74cases of gastric cancer.Meanwhile,18 cases of normal gastric mucosa were servedas normal control.The results showed that cell nuclear DNA aneuploidy incidencewas 4.4% in CAG alone and 25% in CAG with intestinal metaplasia and dysplasiawhich differed significantly from 66.2% in gastric cancer(P<0.01).From normalgastric mucosa to gastric cancer,the SPF and PI increased gradually and remainedstatistically significant difference among various groups(P<0.05 or P<0.01).Theseresults indicated that DNA ploidy,SPF and PI detected by FCM were of value inthe surveillance of precancerous gastric lesions.