血管紧张素Ⅱ1型受体(AT1R)基因多态性与新疆哈萨克族原发性高血压的相关性研究

The study for relationship between the polymorphism of angiotensin Ⅱ type 1 receptor gene A1166C and essential hypertension in kazak of Xinjiang

目的:研究血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态性与新疆哈萨克族原发性高血压(EH)的相关关系。方法: 采用多聚酶链式反应法及限制性片段长度多态性技术(PCR-RFLP),对新疆哈萨克族 198 例EH患者(EH组)及130例正常血压者(对照组)的外周血白细胞DNA进行AT1R基因 A1166C多态性检测,观察AA、AC和CC不同基因型以及该位点A、C不同等位基因频率在EH组和对照组中的分布。结果:AA、AC和CC基因型在EH组的分布频率为0.772 8、0.222 1和0.005 1,在对照组为0.761 5、0.238 5 和 0,两组对比差异无统计学意义(P>0.05); A1166 与 C1166 等位基因频率在高血压组中分别为 0.883 8、0. 116 2,对照组中分别为0.880 8和0.119 2,两组相比差异均无统计学意义(P>0.05)。结论:AT1R基因 A1166C分子变异与新疆哈萨克族原发性高血压无相关关系。

Objective: To investigate the relationship between the A1166C allele polymorphism of angiotensinⅡ Type 1 Receptor (AT1R) gene and the essential hypertension in Kazak of Xinjiang. Methods: Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods were used to detect the A1166C polymorphism of AT1R gene in Kazak including 198 patients with hypertension and 130 normotensive controls. Results: The genotype frequencies of A1166C were AA0.7728, AC0.2221, CC0.0051 in the hypertension group and the corresponding frequencies were 0.7615、 0.2385、 0, in the control group respectively; The A1166 and C1166 allele frequencies were 0.8838、0.1162 respectively in hypertension group and 0.8808、0.1192 in normotensive group; The A1166C-genotype distribution and the allele frequencies of A1166 and C1166 were not statistic significances in hypertension group compared with normotensive group(P>0.05). Conclusion: There was no significant association found between the A1166C polymorphism of AT1R gene and essential hypertension in Kazak of Xinjiang.