Serological survey on canine coronavirus antibodies in giant pandas by virus neutralization test

大熊猫血清犬冠状病毒的中和抗体调查(英文)

In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.

本文为进一步了解犬冠状病毒在大熊猫群体中的感染情况,采用固定病毒(100 TCID50)-稀释血清法建立了检测大熊猫血清CCV抗体的微量中和试验。运用该方法测定了四川大熊猫保护区和动物园的62份大熊猫血清CCV中和抗体,以大于1:4抗体效价作为阳性判定标准,结果8份大熊猫血清为阳性,抗体滴度在1:8和1:32之间,阳性率达12.9%。这是首次比较全面地对大熊猫进行CCV中和抗体水平调查,结果表明: CCV感染在我国大熊猫群体中较为普遍,已给大熊猫的健康带来威胁。因此,有必要研制安全有效的疫苗以保护大熊猫的安全。表1参17。