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Cloning and Sequence Analysis on 3′ Coding Region of Wild Boar and Cross Bred Pig Myostatin Gene

Cloning and Sequence Analysis on 3′ Coding Region of Wild Boar and Cross Bred Pig Myostatin Gene
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摘要 Myostatin, with a highly conservative gene among breeds is a negative regulator of muscle. The 3′ coding regions of wild boar and crossbred pig myostatin were cloned by RT-PCR and sequenced respectively. The homology of the nucleotide sequence between wild boar and crossbred pig was 100% and there was no difference in this region compared with pig myostatin gene of Genbank. This indicated that there was not change of gene sequence in this region during the evolution processes. Myostatin, with a highly conservative gene among breeds is a negative regulator of muscle. The 3′ coding regions of wild boar and crossbred pig myostatin were cloned by RT-PCR and sequenced respectively. The homology of the nucleotide sequence between wild boar and crossbred pig was 100% and there was no difference in this region compared with pig myostatin gene of Genbank. This indicated that there was not change of gene sequence in this region during the evolution processes.
出处 《Journal of Northeast Agricultural University(English Edition)》 CAS 2004年第2期148-150,共3页 东北农业大学学报(英文版)
关键词 野公猪 杂交猪 基因克隆 序列分析 3′编码区 RT—PCR MYOSTATIN基因 myostatin gene RT-PCR 3'coding regions
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参考文献6

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