一种优化的琼脂糖包埋细胞微阵列的制备方法

Optimization of agarose embeded cell microarray

在原有琼脂糖包埋细胞微阵列制备技术基础上 ,建立一种高可靠性和稳定性的细胞微阵列制备方法。方法是收集培养细胞 ,采用优化的低熔点琼脂糖包埋技术包埋目的细胞 ,再对细胞包埋块进行脱水和石蜡包埋 ,最后将细胞石蜡包埋块用组织微阵列仪制备成细胞微阵列。结果显示细胞微阵列切片HE染色细胞结构清晰 ,免疫组化实验后观察阳性信号着色明显 ,定位准确 ,无背景干扰 ,细胞微阵列在 4℃存放 3个月后进行免疫组织化学实验 ,信号无明显衰减。本方法制备的细胞微阵列具有较好的稳定性和可靠性 ,且细胞形态和抗原保存良好 ,可应用于生物科技和医学研究领域各种实验研究。

Based on the current technology of agarose embedded cell microarray, we optimized a preparation method with high dependability and stability. In vitro cultured cells were harvested, and then the target cells were embedded into the low melting point agarose by using an optimized method of dehydration and paraffin embedding. The cell microarrays were made by Tissue Arraying Machine by sectioning the paraffin embedded cell blocks. The resulting in well maintained cell structure were confirmed with H&E stain, and obvious positive signal stain, exact staining place, lack interferential background signal were confirmed with immunohistochemistry staining, the signal attenuation of immunohistochemistry staining was not found after stored for three months. This method for making cell microarray has advantages in the stability and dependability, and the quality of retention of cell structure and maintaining cell antigenecity, it can be applied in the biotechnology and medical research.