微波辅助衍生GC-MS测定脂肪酸及校正变换矩阵法用于食用植物油鉴别的研究

A Microwave-assisted Derivatization and GC-MS Method Coupled with Calibration Transformation Matrix for Determination of Fatty Acid in Edible Oils

建立了微波衍生化GC -MS测定食用植物油中的脂肪酸含量 ,并采用校正变换矩阵法对食用植物油的成分进行测定的方法。对微波衍生化的实验条件进行了优化 ,利用建立的校正模型对40个花生油、菜籽油、芝麻油、棉籽油、棕榈油的二元、三元、四元、五元人工合成样品进行了计算预测 ,所有样品的平均预测误差都小于5 %。对8个实际样品进行了测定 ,其中有3个掺伪样品 ,所测调和食用油的结果与标签标示结果相符性较好。本方法可用于快速、准确地测定食用油中各成分含量或定性。

A microwave-assisted derivatization and GC-MS method coupled with calibration transformation matrix was developed for determination of fatty acid in edible oils.The optimum conditions for microwave-asˉsisted derivatization were investigated in detail.The calibration model was verified with40synthetic samples containing known contents of peanut oil,rape oil,sesame oil,cotton oil,and palm oil.The average deviaˉtions fromthe known values for all samples were less than5%.Eight commercial samples were analyzed with this method,and three products were identified as being adulterated.The results from other samples agreed fairly well with what was given on the labels.This method can be used to determine the contents of edible oils rapidly and accurately,as well as to identify adulterated commercial products.