兔双侧下颌骨牵张成骨实验动物模型的建立

The establishment of an animal model of bilateral mandibular distraction osteogenesis in rabbits

目的:建立兔双侧下颌骨牵张成骨实验动物模型。方法:选用16只成年雄性新西兰大白兔,随机分为4组,每组4只。在双侧下颌骨第一前磨牙前方无牙区行骨切开术,用自制牵张器固定。经过7d潜伏期,以每次0.5mm的速度牵张,每天2次,连续7d。分别在潜伏期末、牵张结束、固定期第14天、第28天处死动物,切取标本行X线和组织学观察。结果:全部动物的下颌骨被成功延长,牵张间隙逐渐被新生骨组织充填。结论:该方法建立的动物模型具有可行性和可重复性。

PURPOSE: To establish an animal model of bilateral mandibular distraction osteogenesis in rabbits. METHODS: 16 adult male white New Zealand rabbits were divided into 4 groups randomly, 4 in each group. Osteotomy was performed on mandible bilaterally before the first premolar. Bone stumps were repositioned and fixed with distractors. After 7 days of latency period, distraction was initiated at a rate of 1mm/d for 7 days. Then distractors were held in place for days. Animals were killed at different time points, and specimens were taken for X-ray and histology examination. RESULTS: The mandibles were lengthened successfully. The distraction gaps were filled with regenerated bone tissue. CONCLUSION: The method in this study is practicable and repeatable which could be used to establish an animal model of mandibular distraction osteogenesis in rabbits.