小型猪软腭射频治疗后的组织学及超微结构观察

Histological and ultrastructural variations after radiofrequency for soft palate in porcines

目的:建立软腭射频治疗的动物模型,观察软腭经射频治疗后的组织学及超微结构改变。方法:巴马小型猪13只,分为实验组和对照组。实验组10只,在软腭中线的中份给予射频能量2.4KJ,对照组仅在相同部位插入射频针,不给予能量,在72h、2周、4周处死动物;实验组在治疗后1h、24h、48h、72h、1周、2周、3周、4周、6周、9周处死。取软腭组织,在光镜和电镜下观察软腭射频后的组织学及超微结构改变。结果:射频后1～72h,组织呈炎性变化,血管扩张充血,大量炎细胞浸润;射频后1周,大量成纤维细胞和血管增生,并随时间推移,受损组织逐渐被胶原纤维替代。射频作用区周围的组织呈可逆性变化。结论:软腭射频治疗后,最终胶原纤维修复受损组织,且仅限于作用区,这为临床安全治疗患者提供了理论依据。

PURPOSE: To investigate the histological and ultrastructural variations after radiofrequency volumetric reduction of the soft palate in an animal model. METHODS: Thirteen porcines were used to evaluate the tissue response to radiofrequency for various time periods. They were divided into two groups. Group 1 was exposed to radiofrequency in the midline of the soft palate with a constant energy of 2.4KJ. Group 2 served as a control group. The animals in group 1 were sacrificed after 1 hour, 24 hours, 48 hours, 72 hours, 1 week, 2 weeks, 3 weeks, 4 weeks, 6 weeks and 9weeks, respectively, and after 72 hours, 2 weeks and 4 weeks for the animals in group 2. Then the soft palates from both groups were examined for histological and ultrastructural variations. RESULTS: Interstitial edema, hemorrhage and infiltration with inflammatory cells were observed in the early acute stage after radiofrequency, and then the neovascularization of the forming scar was observed. In the end, the injuried tissue was replaced by collagenous fibers. Intact vessels and nerves were observed around the lesions. CONCLUSIONS: After radiofrequency, lesion tissue is replaced by collagenous fibers, and it is focused on the lesion site. These findings may help provide a basis for technological suggestion in regard to clinical treatment.