摘要
合成了雌酚酮—卟啉及其钴络合物,研究了其催化氧化异丙苯的动力学,用Lineweave-Burk法求得动力学参数V_(max)=5.506×10^(-3),K_m=6.081×10^(-2)和K_2=25.37。
The estrone-porphyrin and estrone-cobaltioporphyrin complex have been synthesized, and with the latter as enzyme model,the catalytic activity for oxidizing the isopropylbenzene into acetophenone in DMF has been studied in detailed at 35℃. The kinetic parameters of the classical MichaclisMenten equation by the Lineweave-Burk plot are given by V+max= 5.506×10_-3 (tool.l_-1·rain_-1). K_m =6.081×10_-2,and K_2=25.37(rain_-l)correlation coefficient (r) is 0.9985. From value of turnover number (T.N),it can be seen that the estrone-cobaltioporphyrin is close to that of the lysozyme.
出处
《天然产物研究与开发》
CAS
CSCD
1993年第4期15-21,共7页
Natural Product Research and Development
基金
国家教委资助优秀年轻教师基金
四川省青年基金
关键词
酶模型
催化
动力学
雌甾金属叶啉
estrone-cobaltioporphy-rin ,enzyme model,catalytic kinetics
