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转基因植物表达植酸酶研究进展 被引量:12

Research Progress in Transgenic Plants Expressing Recombinant Phytase
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摘要 植酸是植物体内磷的主要存在形式,其绝大部分不能被单胃动物消化吸收,而随粪便排出体外造成环境污染;同时,植酸又是一种抗营养因子,它通过络合植物体内的一些营养成分而降低植物的营养价值。通过植物转基因方法使植物自身表达足量的植酸酶,以减小植酸带来的不利影响,是提高植物性饲料营养价值和控制环境磷污染的一种经济有效的措施。就转基因植物植酸酶的优势、研究现状、存在的问题及其发展前景进行了综述。 Phytic acid is the main storage form of phosphorus in plants. Due to the lack of phytase in monogastric animal digestive tract, most of the phytic acid is unavailable, and directly excreted to manure. High phytic acid content in manure results in elevated phosphorus levels in soil and water and accompanying environmental concerns. Furthermore, as a sort of antinutrient, phytic acid will chelated some nutrients in plants and consequently reduce the nutrition value of plants. It is desirable that transgenic plants themselves express sufficient recombinant phytase to minimize the disadvantage from phytic acid by plants gene engineering. The superiority of transgenic plants phytase is summarized, the research progress is reviewed, and its development prospects are also discussed.
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2005年第1期29-32,共4页 China Biotechnology
基金 国家自然科学基金资助项目(30370889)四川省生物技术攻关项目
关键词 转基因植物 植酸酶 植酸磷 植酸 转基因模式植物 转基因饲料作物 Transgenic plants Phytic acid Phytase
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参考文献21

  • 1袁文杰,张苓花,王晨祥,王运吉.植酸酶植物基因工程载体的构建[J].大连轻工业学院学报,2000,19(4):264-267. 被引量:1
  • 2鲁晓岩,钱方,张苓花,王运吉.无抗性选择标记的植酸酶基因转化DNA的构建[J].大连轻工业学院学报,2002,21(2):120-123. 被引量:1
  • 3Verwoerd T C, van-Paridon P A, van-Ooyen A J, et al. Stable accumulation of Aspergtgillus niger phytase in transgenic tobacco leaves. Plant Physiol, 1995, 109:1199-1205.
  • 4Yip W, Wang L J, Cheng C W, et al. The introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco. Biochemical and Biophysical Research Comnamications, 2003, 310:1148-1154.
  • 5Coello P, Maughan J P, Mendoza A, eL al. Generation of low phytic acid Arabidopsis seeds expressing an E. coli phytase during embryo development. Seed Science Research, 2001, 11:285-291.
  • 6Richardson A E, Hadobas P A, Hayes J E. Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate. The Plant Journal, 2001, 25:641- 649.
  • 7Mudge S R, Smith F W, Richardson A E. Root-specific and phosphate-regulated expression of phytase under the control of a phosphate transporter promoter enables Arabidopsis to grow on phytate as a sole P source. Plant Science, 2003, 165:871-878.
  • 8Li J, Hegeman C E, Hanlon R W, et al. Secretion of active recombinant phytase from soybean cell-suspension cultures. Plant Physiol, 1997, 114: 1103-1111.
  • 9Hegeman C E, Grabau E A. A novel phytase with sequence similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings. Plant Physiol, 2001, 126:1598-1608.
  • 10Brinch-Pedersen H, Olesen A, Rasmussen S K, et al. Generation of transgenic wheat (Triticum aestivum L. ) for constitutive accumulation of an Aspergillus phytase. Molecular Breeding,2000, 6:195-206.

二级参考文献5

  • 1[1]JONE J D G. Pro-ferential transposition of the maize element ac ivat or in linked chromosomal location in tobacco[J]. Plant cell,1990,(2): 701-707 .
  • 2[2]HAN Yanming, LEI Xingen.Role of glycosylation in the function express ion of an aspergillus niger phytase in pichia pastoris[J]. Archieve of Bioche mistry and Biophysics,1999,(364): 83-90.
  • 3[3]RUSSEL S H, HOOPES J L. Directed excission of transgene from th e plant genome[J]. Mol Gen Genet,1992,(234):49-59.
  • 4[4]SHINIZU M. Punification and characterisation of phytase from Ba cillus subtilis cnatto N-17[J]. Biosci Biotechnol Biochem, 1992,(56): 1 266 -1 269.
  • 5[5]GREEN I M. A chronomosome replication pattern deduced from percar p phenotypes resultin from movements of the transposable element[J]. Moclulat or in Maize, 1984,(108): 471-485.

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