血管紧张素Ⅱ对动脉粥样硬化斑块炎症的影响

Effect of Atherogenic Process by Angiotensin

目的探讨血管紧张素Ⅱ(AⅡ)对动脉粥样硬化(AS)斑块炎症及稳定性的影响。方法新西兰兔12只,随机分为单纯高脂组(注射生理盐水)和AⅡ组(注射AⅡ)各6例,喂养3个月。采用计算机图象分析系统测定内膜、中膜的厚度和面积,免疫组化法检测血管壁巨噬细胞表达。结果AⅡ组内膜、中膜的厚度和面积明显高于单纯高脂组水平(P<0.05);AⅡ组AS斑块内含有大量巨噬细胞和巨噬细胞源性泡沫细胞,而单纯高脂饮食组AS斑块内只有稀疏分布的巨噬细胞和巨噬细胞源性泡沫细胞。结论注射AⅡ能明显促进高脂饮食兔AS斑块的炎症程度,加重其病理过程。

Objective To explore the relationship between plasma concentration of angiotensin Ⅱ(AⅡ) and inflammation and vulnerable plaque in atherosclerosis ( AS ). Methods AS was induced in the rabbit aorta by atherogenic diet for 3 months; meantime, animals were randomized to receive either vehicle or AⅡ. The intimal areas and medial areas in the aorta were analysed by computer-based color image analysis. Macrophages were detected by immunohistochemistry. Results The intimal areas and medial areas in the aorta were significantly higher in AII group than those in vehicle group(P <0.05). The AⅡ -induced lesions contained predominantly macrophages, while the atherosclerotic lesions of vehicle group contained scanty macrophages. Conclusion AⅡ provoked marked proinflammatory response in vascular pathology and promoted an increased severity of aortic atherosclerotic lesions in rabbits taking atherogenic diet.