肌苷对帕金森病模型小鼠行为学及纹状体多巴胺水平的影响

Effects of inosine on praxiology and the level of dopamine in striatum of mice with Parkinson disease

目的:观察肌苷对1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)致帕金森病模型小鼠行为学及多巴胺水平的影响。方法:实验在解放军第四军医大学实验动物中心实验室和基础部神经生物教研室实验室进行。取雄性C57BL小鼠20只,腹腔注射MPTP建立C57BL小鼠帕金森病模型,随机分为生理盐水组和肌苷组各10只,通过行为学检测、免疫组织化学和荧光分光光度法,观察肌苷对小鼠帕金森病模型的行为学表现、黑质多巴胺神经元和纹状体酪氨酸羟化酶免疫反应阳性(tyrosinehydroxylase-immunoreactive,TH-ir)神经纤维以及纹状体多巴胺水平的影响。结果:给予肌苷后帕金森病小鼠行为学计数和黑质多巴胺神经元数目与生理盐水组相比差异无显著性意义,但纹状体TH-ir神经纤维密度和纹状体多巴胺水平升高,给予肌苷后纹状体多巴胺水平是生理盐水组的2.08倍。结论:肌苷对MPTP所致的帕金森病模型小鼠具有改善自主活动的能力,并且对纹状体内多巴胺水平也产生干预效应。

AIM:To study the effect of inosine on the praxiology and the level of dopamine in siriatum of mice with Parkinson disease(PD) induced by 1 methyl 4 phenyl 1, 2,3,6 tetrahydropyridine(MPTP). METHODS:The study was finished in Laboratory of Animal Center and Department of Neurobiology, Fourth Military Medical University of Chinese PLA.The PD model were formed with intraperitoneal injections of MPTP in twenty male adult C57BL mice. Then the twenty MPTP induced PD mice were divided into saline group(10 mice) and inosine group(10 mice) randomly.Inosine was administered intraperitoneally to mice.The effect of inosine on behavioral manifestation, the number of dopamine neurons in the substantia nigra and density of tyrosine hydroxylase immunoreactive(TH ir) nerve fibers in the striatum,and the level of dopamine in the striatum were observed by behavioral test,immunohistochemical analysis and fluorospectrophotometry. RESULTS:Compared with saline group,there was no significant difference in the behavioral activity and number of dopamine neurons in the substantia nigra in inosine group. But the density of TH ir nerve fibers and level of dopamine in the striatum were higher in inosine group.The level of dopamine in the striatum in inosine group was 2.08 times higher than that in saline group. CONCLUSION:Inosine exerts a therapeutic effect on MPTP induced PD in C57BL mice.