低剂量氦氖激光血管内照射对大鼠脑缺血再灌注的影响(英文)

Effects of intravascular low dose He-Ne laser irradiation on rats after ischemia reperfusion

背景:许多体外实验研究表明低剂量氦氖激光可刺激细胞生长和促进血管再生,从而改善缺血所致的损害,而体内试验需进一步研究。目的:研究低剂量氦氖激光血管内照射对大鼠脑缺血再灌注的保护作用,探讨其作用机制。设计:以实验动物为研究对象,随机对照研究。单位:一所大学医院的神经内科。材料:实验于2003-09/2004-02在四川大学华西医院外科动物实验中心完成。健康雄性SD大鼠,二级动物,五六月龄,体质量372～418g(388.48±10.57)g,由四川大学动物研究中心提供。干预:采用大鼠大脑中动脉缺血(MCAO)模型,将40只Sprague-Dawley(SD)大鼠随机分成治疗组和对照组,每组20只。治疗组术后予以低剂量氦氖激光(2mW)血管内照射,20min/次,隔日1次,3次为1个疗程。对照组同样予以静脉穿刺,但无激光输出。主要观察指标:脑梗死体积比。结果:低剂量氦氖激光组血管内照射组脑梗死体积比10.43±1.04,明显低于对照组16.78±1.12,差异有统计学意义(t=27.14,P<0.05)。结论:低剂量氦氖激光血管内照射对大鼠脑缺血有保护作用。

BACKGROUND:Quite a number of in vitro experiments indicate that low dose He Ne laser can stimulate the cell growth and blood vessel regeneration therefore to improve the damages caused by ischemia. However,it needs further study on the in vivo experiments. OBJECTIVE:To study the protective effects of intravascular low dose laser irradiation to ischemic reperfusion of rats and explore its mechanism. DESIGN:A randomised and controlled trial based on experimental animals. SETTING:Department of Neurology of a hospital of a university. MATERIALS:The experiment was completed in the Surgery Experimental Animal Center of West China Hospital of Sichuan University.Healthy male SD rats of class II which aged between 5 to 6 months with weight of 372 to 418 grams[mean weight(388.48±10.57) g]were provided by Animal Research Center of Sichuan University. INTERVENTIONS:Middle cerebral artery ischemia model of rats was prepared.Forty SD rats were randomly divided into treatment group and control group with each of 20 rats.Intravascular low dose He Ne laser irradiation was conducted to rats in treatment group one time every other day,20 minutes per time and 3 times for a course.Vein puncture was conducted to rats in control group without laser input. MAIN OUTCOME MEASURES:Ratio of infarcted volume RESULTS:The ratio of the cerebral infarction volume in treatment group was(10.43±1.04) and is much lower than that of control group(16.78±1.12). There was statistical difference between two groups(t=27.14,P< 0.05). CONCLUSION:Intravascular low dose He Ne laser irradiation has protective effects to cerebral ischemia of rats.