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人骨髓间充质干细胞缝隙连接通讯功能的荧光漂白恢复法测定

Gap junctional intercellular communication of human mesenchymal stem cells detec ted by fluorescence redistribution after photobleaching
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摘要 目的:研究脉冲电磁场(pulsedelectromagneticfields,PEMF)对人骨髓间充质干细胞(humanmesenchymalstemcells,hMSCs)缝隙连接所介导的细胞通讯(gapjunctionalintercellularcommunication,GJIC)的影响。方法:实验于2004-07/09在解放军第三军医大学中心实验室完成。应用荧光漂白恢复(fluorescenceredistributionafterphotobleaching,FRAP)技术,通过激光共聚焦显微镜检测hMSCs经PEMF刺激后的GJIC功能变化。结果:经PEMF刺激后的hMSCs,平均荧光漂白恢复率为(64.12±0.83)%,对照组为35.26±0.76)%,前者较后者有显著性增加((t=-15.49,P<0.01)。结论:PEMF刺激能促进hMSCs的缝隙连接通讯功能。 AIM:To investigate the influence of irradiation of pulsed electromagnetic fiel ds(PEMF) on the gap junctional intercellular communication(GJIC) of human mesenc hymal stem cells(hMSCs). METHODS:The experiment was completed in the central laboratory of Third Milita ry Medical University of Chinese PLA from July to September 2004.Fluorescence re distribution after photobleaching(FRAP) was used to evaluate the functional chan ges of GJIC under a laser scanning confocal microscope after the MSCs were irrad iated by PEMF. RESULTS:The mean fluorescence recovery rates of the hMSCs irradiated by PEMF[( 64.12±0.83)%]was significantly higher than that of the control group[(35.26±0 .76)%](t=-15.49,P< 0.01). CONCLUSION:PEMF can accelerate the GJIC of hMSCs.
作者 宋晋刚 周强 许建中 罗飞 孙玮
机构地区 解放军第三军医大学西南医院骨科 解放军第三军医大学高原军事医学系中心实验室
出处 《中国临床康复》 CSCD 北大核心 2005年第2期78-79,i003,共3页 Chinese Journal of Clinical Rehabilitation
基金 国家高技术研究发展计划(八六三)重大专项2003AA205020) 重庆市(科技攻关项目(2003-13-6) 西南医院创新基金(2002-15)~~
关键词 缝隙连接 细胞通讯 脉冲电磁场 间充质干细胞
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献10

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2005年 第2期

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