骨髓间充质干细胞复合胶原构建组织工程皮肤修复皮肤缺损

Construction of tissue engineered skin by bone marrow mesenchymal stem cells wit h collagen for repair of skin defect

目的:骨髓间充质干细胞(mesenchymalstemcells,MSCs)具有多向分化潜能和持续增殖能力,显示出作为组织工程种子细胞新来源的诱人前景。探讨MSCs作为皮肤组织工程种子细胞修复皮肤缺损的可能性。方法:采用密度梯度离心结合贴壁法分离纯化新西兰兔MSCs,经体外扩增后接种于胶原凝胶构建组织工程活性皮肤替代物。12只兔随机分成3组:MSCs复合胶原组织工程活性皮肤替代物实验组、单纯胶原对照组和空白对照组。将各组相应材料分别移植于兔耳皮肤缺损处,比较观察创面修复效果。结果:MSCs复合胶原实验组、单纯胶原对照组和空白对照组创面愈合时间分别为(14.2±1.9)d,(16.3±2.7)d和(20.1±4.1)d,差异有显著性意义(P<0.05)。组织学观察显示,MSCs复合胶原实验组成纤维细胞、微血管、胶原纤维均较单纯胶原对照组和空白对照组明显增多。结论:MSCs复合胶原构建的组织工程活性皮肤替代物移植后可加速新生血管形成、促进创面修复。提示以MSCs作为种子细胞复合胶原构建的组织工程皮肤可用于皮肤缺损的修复治疗。

AIM: Bone marrow mesenchymal stem cells(MSCs) have the ability of the multipot ential differentiation and extended proliferation, which suggests these cells as an attractive cell source for tissue engineering. The aim of this paper is to p robe into the feasibility of MSCs as the seeding cells of skin tissue engineerin g for the repair of skin defect. METHODS: MSCs were separated from bone marrow of New Zealand rabbits, and puri fied by combining density gradient centrifugation with adhering method. The ampl ified cells in vitro were inoculated in collagen gel to form tissue engineered s kin. Twelve rabbits were randomly divided into three groups: experimental group of MSCs with collagen tissue engineered active skin substitute, single collagen control group and blank control group. The materials were grafted onto total ski n defect wounds on the ears of the rabbits in the corresponding groups respectiv ely. The effect of wound repair was observed. RESULTS: The time of wound healing in the MSCs with collagen experimental grou p, the single collagen control group and the blank control group were(14.2±1.9) days,(16.3±2.7)days and (20.1±4.1) days respectively, which have significant differences (P< 0.05). Histological observation showed that the fibroblasts, mic ro vascularities and collagen fibers in the MSCs with collagen experimental gro up were significantly increased as compared with the single collagen group or th e blank control group. CONCLUSION:MSCs with collagen can accelerate the formation of fresh blood vess els of the wounds and promote the wound repair.It suggests that MSCs with collag en maybe an optional method of skin reconstruction for wound repair.