高强度聚焦超声热疗法对原发性肝癌患者T淋巴细胞亚群和NK细胞的影响

Effect of high intensity focused ultrasound on T-lymphocyte subsets and natural killer cells in patients with primary hepatocellular carcinoma

目的:探讨高强度聚焦超声热疗(highintensityfocusedultrasound,HIFU)对原发性肝癌患者免疫功能的影响。方法:泰安市中心医院肿瘤内科2002-10/2004-04原发性肝癌患者42例。分别取42例肝癌患者HIFU治疗前和治疗后4周外周血2mL,用流式细胞仪检测NK细胞,CD4+,CD8+T细胞百分数和CD4/CD8。结果:经HIFU治疗后肝癌患者的NK细胞百分数显著升高(t=7.475,P<0.05),CD4+T细胞百分数和CD4/CD8比值明显升高t=2.272,P(<0.05;t=3.216,P<0.01),CD8+T细胞百分数则下降(t=2.148,P<0.05)。结论:应用HIFU技术的物理热疗法可以改善肝癌患者体内的细胞免疫功能。

AIM:To study the heat treatment of high intensity focused ultrasound(HIFU) on the immune function of patients with primary hepatocellular carcinoma(HCC). METHODS:From October 2002 to April 2004,42 patients with primary HCC were sele cted from the Department of Oncology,Taian Central Hospital.The 42 patients were drawn with 2 mL peripheral venous blood,and then the percentage of natural kill er(NK) cells and T lymphocyte subsets such as of CD4+,CD8+as well as CD4/CD8 were examined before and 4 weeks after the heat treatment of HIFU. RESULTS:After the treatment of HIFU,the percentage of NK was significantly dec reased(t=7.475,P< 0.05),the percentage of CD4+and the ratio of CD4/CD8 were enh anced significantly(t=2.272,P< 0.05,t=3.216,P< 0.01,respectively).The percentage of CD8+was significantly lower than those of patients before treatment(t =2.14 8,P< 0.05). CONCLUSION:The heat treatment of HIFU can improve the cellular immune function of patients with primary HCC.