抑癌基因PTEN和P53在骨肉瘤组织中的表达及对预后的影响

Expression of PTEN and P53 in osteosarcoma and their effect on prognosis

目的:研究骨肉瘤组织中抑癌基因PTEN和P53的表达情况,及其在骨肉瘤的发生、发展中的可能作用。方法:收集中国医科大学附属第一医院1995-05/2002-11骨肉瘤标本38个及骨软骨瘤标本20个。用免疫组织化学SP法检测PTEN及P53在38个骨肉瘤及20个骨软骨瘤石蜡标本中的表达情况。结果:PTEN蛋白在骨肉瘤中表达阳性率为55%(21/38),在对照组骨软骨瘤中为90%18/20),骨肉瘤与骨软骨瘤相比差异有显著性意义((χ2=4.62,P<0.05)。P53在骨肉瘤中表达阳性率为66%(25/38),在对照组骨软骨瘤中为5%(1/20),骨肉瘤与骨软骨瘤相比差异有非常显著性意义(χ2=19.58,P<0.01)。结论:抑癌基因PTEN和P53在骨肉瘤组织中表达下调,可能与骨肉瘤的恶性进展有关。

AIM:To study the expression of anti oncogene PTEN and P53 in the occurrence a nd development of osteosarcoma. METHODS:Expressions of PTEN and P53 were detected with SP immunohistochemical method in 38 paraffin embedded samples of osteosarcoma and 20 of osteochondroma collectd from the First Affiliated Hospital of China Medical University from Ma y 1995 to November 2002. RESULTS:The positive rate of PTEN expression was 55%(21/38) in osteosarcoma a nd 90%(18/20) in osteochondroma. The difference between them was significant(χ 2=4.62,P< 0.05).The positive rate of P53 expression was 66%(25/38) in osteosarc oma and 5%(1/20) in osteochondroma. There was a significant difference between them(χ2=19.58, P< 0.01). CONCLUSION:The down regulation of PTEN and P53 expression may relate to the m alignant development of osteosarcoma.