菜籽多糖的抗氧化作用与机理研究

Study on the Anti-Oxidative Effect and Mechanism of Polysaccharide from Rapeseed(RSPS)

研究菜籽多糖粗品的抗氧化作用与机理。用K3[Fe(CN)6]与TCA体系、脂肪氧合酶评价菜籽多糖的还原能力及抑制脂肪氧合酶的能力,用丙二醛(MDA)及活性氧(ROS)试剂盒测定MDA及ROS含量,用分光光度法测定小鼠肝线粒体肿胀度。结果显示,在化学模拟体系中,菜籽多糖具有还原能力,2.00mg·ml-1菜籽多糖的抗活性氧单位为94.03,对脂肪氧合酶的抑制率达22.8%。在体外,2.00mg·ml-1菜籽多糖使自由基诱导的小鼠肝线粒体肿胀度低于非诱导组,对自由基诱导的小鼠肝线粒体MDA生成抑制率达30.3%,对肝组织匀浆MDA生成抑制率分别为54.7%(非诱导组)、32.0%(Fe2+诱导组)及84.5%(H2O2诱导组)。10.00mg·ml-1菜籽多糖使小鼠血清抗活性氧单位达1340.13。在体内,腹膜注射菜籽多糖组小鼠(400mg·kg-1bw·d-1,12d)与对照组相比,肝匀浆MDA的降低达到极显著水平(P<0.01)。以上结果表明,菜籽多糖在体内外均具有明显的抗氧化作用,菜籽多糖具有还原能力及抑制与氧化有关的酶的活性可能是其抗氧化的机理。

The anti-oxidative effect and mechanism of RSPS were studied. The K3[Fe(CN)6], TCA system and the crude lipoxygenase were used to evaluate RSPS’s capacity of deoxidizing and its inhibition on lipoxygenase. The reagent kits were used to analyse the content of malondialdehyde(MDA) and reactive oxygen species (ROS), and the swelling of mice liver mitochondria was observed in the spectrophotometric method. The results showed that RSPS was a good reducer, and the anti- ROS unit of 2.00 mg·ml-1 RSPS was 94.03, and the inhibiting rate of 2.00 mg·ml-1 RSPS on lipoxygenase was 22.8% in some chemically modified systems. In vitro, 2.00 mg·ml-1 RSPS made the radical-induced swelling of mice liver mitochondria be lower than the swelling of mice liver mitochondria without inducement. The inhibiting rate of 2.00 mg·ml-1 RSPS on radical-induced MDA formation was 30.3% in mice liver mitochondria, while 54.7%(incubation), 32.0%(incubation with Fe2+) and 84.5%(incubation with H2O2) in liver homogenate, respectively. The anti- ROS unit of mice serum, added with 10.00 mg·ml-1 RSPS, was 1340.13. In vivo, there was a significant difference(P<0.01) between the controlled group and the intra- peritoneal injected group (with RSPS 400mg·kg-1 bw·d-1, 12 d) in MDA value in mice liver homogenate. All the results above showed that RSPS has anti-oxidation effect in vitro and in vivo, the high capacity of deoxidising and inhibiting the activity of enzymes associated with oxidation maybe the possible mechanism of RSPS to inhibit oxidation.