摘叶对大樱桃休眠花芽内源激素及活性氧代谢的调控

Effects of Defoliation on Endongenous Hormones and Active Oxygen Metabolism of Sweet Cherry Flower Buds During Dormancy

以8年生大樱桃为试材,分析了人工摘叶处理对休眠花芽中内源激素含量,活性氧物质H2O2、O2·含量及抗氧化酶SOD、POD、CAT活性的影响,同时研究了摘叶打破休眠的效应。结果表明,与对照相比,摘叶后花芽各种激素均出现不同程度的下降,其中,ABA下降最显著。摘叶后期GA3和ZT等生长激素的含量逐渐回升,且明显高于对照花芽。摘叶增加了花芽中的H2O2含量,摘叶后30d较同期对照增加了34.57%,而O2·含量出现-降低的趋势,摘叶后30d比同期对照降低了25.49%。另外,摘叶提高了SOD的活性,而CAT活性降低,这与H2O2含量的降低一致,摘叶同时还提高了POD的活性。摘叶花芽平均较对照提早萌芽3～6d,提早2～4d解除休眠。

Effects of picking off leaves on endogenous hormones, H2O2, O2 content, SOD, POD, CAT activities in 8-year-old -. sweet cherry flower buds during dormancy were investigated, and the effect of picking off leaves on dormancy release was also studied. Results indicated that four kinds of endogenous hormones contents in sweet cherry leaves all dropped after picking off leaves, especially ABA content. GA3 and zeatin content increased steadily during upper stage of picking off leaves and were remarkably higher than the control. Meanwhile, H2O2 content in flower buds increased after picking off leaves, it increased by 34.57% compared with the control after picking off leaves for 30 days. However, O2 ccontent decreased by 25.49% compared with -. the control. Moreover, SOD activity increased and CAT activity decreased after picking off leaves, which were consistent with the reduce of H2O2 content. POD activity increased after picking off leaves. At last, flower buds after picking off leaves germinated 3-6 days earlier than the control, and released dormancy 2-4 days earlier than the control.