PVY^N与PVY^O病毒RT-PCR快速检测体系研究

Studies on the Methods of Detecting PVY^N and PVY^O by RT-PCR

马铃薯Y病毒(PVY)是侵染马铃薯的重要病毒之一,严重影响马铃薯的生产。本文通过对PVY病毒N株系(PVYN)与O株系(PVYO)的基因序列进行比较,在其外壳蛋白同源区设计一对通用引物,建立了PVY病毒的RT-PCR检测体系。另外在N株系与O株系同源区设计一条3′引物,选择N株系与O株系差异区设计两条5′端引物,建立了可鉴定PVYN与PVYO的复合RT-PCR体系。这个体系的建立,对于马铃薯脱毒苗的检测与PVY病毒的调查及病理研究都具有一定的应用价值。

Potato virus Y(PVY) is one of the important viruses infecting potatoes. It influences the production seriously. The nucleotide sequences of potato virus Y N-strain(PVYN)and O-strain(PVYO) were compared. A pair of homologous primers were designed according to the conserved region of coat protein gene. So the RT-PCR method of detecting PVY was built. Judging by the result of comparison, the 3′primer was designed in conserved region and two 5′primers were designed in two variable region. So a complex RT-PCR method was built which could distinguish PVYN and PVYO. The two methods were very powerful for detecting virus-free seed potatoes, investigating PVY and studying its pathology.