五倍子水提取物对PgLPS诱导单核细胞分泌IL-6的抑制作用

Inhibitory effect of Chinese nutgall extracts on the secretion of IL-6 from mon ocytes induced by lipopolysaccharide of Porphyromonas gingivalis

actsont目的 :研究五倍子水提取物对牙龈卟啉菌 (Pg)内毒素 (LPS)介导的人单核细胞分泌IL 6水平的影响。方法 :采用健康人外周血分离培养的单核细胞以 2 5 μg/ml牙龈卟啉菌内毒素作为刺激因子 ,用放射免疫分析法 (RIA)测定细胞培养上清中IL 6的水平 ,观察 5种浓度五倍子水提取物对单核细胞分泌炎性细胞因子的影响 ,结果 :五倍子水提取物可显著抑制牙龈卟啉菌内毒素诱导人单核细胞分泌IL 6的水平 ,其作用在一定范围内呈浓度依赖性。结论 :五倍子水提取物能够显著抑制牙龈卟啉菌内毒素诱导人单核细胞分泌IL 6的水平 ,提示五倍子具有一定的抗炎作用 ,有助于对牙周病的防治。

Objective: To observe the effects of Chinese nutgalls ex tr acts on the secretion of IL-6 from monocytes induced by lipopolysaccharide (LPS ) of Porphyromonas gingivalis (Pg). Methods:Monocytes ob tained by separating human blood cells were stimulated with LPS of Pg at 25 μg/ml, radioimmunoassay (RIA) was used to examine the effect of Chinese nutgal ls extracts at the concentrations of 6.25-100 μg/ml on the secretion of IL-6 in the supernatant of the cell culture. Results:Chinese nutgalls extracts at the concentrations(μg/ml) of 6.25,12.50, 25.00 ,50.00 and 100.00 inhibited IL-6 secretion from monocytes by(%) 34.6,46.4,48.5,52.7 and 54.9 res pectively.Conclusions:Extracts from Chinese nutgalls may inhibit the secretion of IL-6 from monocyte induced by LPS of Pg in a dose-depend ent manner.