HPLC同时测定新复霜中氯霉素和倍他米松的含量

Simultaneous determination of chloramphenicol and betamethasone in Xinfu cream by HPLC

目的 建立新复霜中氯霉素和倍他米松的含量测定方法。方法 采用HPLC法 ,色谱柱为 μBondapakC18(10 μm ,3.9mm×30 .0mm) ,流动相为甲醇 - 0 .0 2 5mol·L-1NaH2 PO4(6 5∶35 ) ,检测波长 2 4 0nm ,流速 1 2ml·min-1,柱温 4 5℃ ,灵敏度 0 .0 2AUFS。结果 氯霉素的线性范围为 2 0 0 .0～ 5 0 0 .0mg·L-1,平均回收率为 10 .3 2 % (RSD为 0 . 83%～ 2 .17% ) ;倍他米松的线性范围为2 1.0～ 5 2 .5mg·L-1,平均回收率为 10 0 .8% (RSD为 1.11%～ 2 .6 4 % )。结论 所用方法简便、准确 ,适合新复霜的质量控制。

OBJECTIVE To establish a method for the determination of chloramphenicol and betamethasone in Xinfu cream. METHODS An RP-HPLC method was developed. Determination was performed on μBondapak C 18 column(10 μm,3.9 mm×300 mm) with methanol-0.025 mol·L -1NaH 2PO 4(65∶35) as the mobile phase. The flow rate was 1.2 ml·min -1, and the wavelength of detection was 240 nm. The column temperature was set at 45℃. RESULTS The linear range were 200.0-500.0 mg·L -1 and 21.0-52.5 mg·L -1 and the average recovery was 103.2% and 100.8% for chloramphenicol and betamethasone, respectively. CONCLUSION This method is simple and accurate. It can be used for the quality control of Xinfu cream.