荧光定量PCR法检测外周血淋巴细胞中EB病毒DNA含量对鼻咽癌诊断及预后的价值

Clinical application of fluoroquantitative analysis of lymphocyte Epstein-Barr virus DNA by PCR to diagnosis and prediction of prognosis of nasopharyngeal carcinoma.

目的 评价外周血淋巴细胞中EB病毒DNA含量对鼻咽癌诊断及预后的价值。方法 采用荧光定量PCR方法检测 52例鼻咽癌患者和 127例非鼻咽癌患者外周血淋巴细胞中EB病毒DNA含量。结果 鼻咽癌患者中EB病毒DNA的阳性率为 55 76% (29 /52),对照组阳性率为 8 66% (11 /127),阳性率差异有显著性P<0. 01。早期(Ⅰ、Ⅱ期)鼻咽癌患者EB病毒DNA含量与晚期 (Ⅲ、Ⅳ期 )比较有显著性差异(P<0 05)。结论 荧光定量PCR方法检测外周血淋巴细胞中EB病毒DNA含量在鼻咽癌的诊断上有极高价值,可作为判断疗效及监测病情的一种有效手段。

Objective To evaluate the ro1e of Epstein-Barr virus(EBV)DNA in peripheral lymphocyte in diagnosis and predication of patients with nasopharyngeal carcinoma (NPC). Peripheral lymphocyte EBV-DNA in 52 cases of NPC and 127 cases of non-NPC were analysed by fluoroquantitative Polymerase Chain Reaction(PCR).Results The positive rate of lymphocyte EBV-DNA in NPC group was 55.76%(29/52)and 8.66%(11/127) in the contro1 group,the percent of postive in the fomer was significant higher than the later,P<0.01.EBV-DNA concentration of patient with NPC in early stage(phase I、Ⅱ)was lower than 1ater stage(Ⅲ、Ⅳ),P<0.05.Conclusion Fluoroquantitative analysis of peripheral lymphocyte EBV-DNA may be a valuble technique for diagnosing patients with NPC and predicting the prognosis.