共聚焦显微镜观察凋亡相关基因Fas和FasL在肝外胆管癌中的表达与其病理分级的关系

Expression of Fas and FasL gene in extrahepatic cholangiocarcinoma and the relation between it and their pathological grades observed under the laser scanning confocal microscope.

目的 研究细胞凋亡相关基因Fas和FasL在肝外胆管癌组织中的表达及意义。方法 采用免疫荧光组织化学方法结合激光扫描共聚焦显微镜观察。结果 30例肝外胆管癌中Fas和FasL阳性 21例,阳性率 70%,用于对照的 10例胆总管囊肿Fas和FasL阳性l例,阳性率 10%,Fas和FasL在肝外胆管癌中的表达强于对照组(P<0. 05);高中分化癌Fas和FasL定量大于低分化癌 (P<0. 05)。结论 Fas和FasL不同程度地参与了大部分肝外胆管癌的凋亡调变,其表达与肝外胆管癌的分化有关。

Objective To study the expression and significance of proapoptosis gene Fas and FasL in extrahepatic cholangiocarcinoma.Method Immunofluorescent histochemical double-staining technique was used.The staining results were observed under the laser scanning confocal microscope.Results Fas and FasL immunoreactivity was detected in 21 out of 30 cases of extrahepatic cholangiocarcinoma (ECHC) and 1 out of 10 cases of choledochocele;Expression of Fas and FasL in ECHC was higer than that in choledochocele (P<0.05).The quantity in well and moderately differentiated tumor was larger than that in poorly differentiated.Conclusion The results suggest that Fas and FasL are expressed in most of ECHC.Fas and FasL may take part in the modulation of apoptosis in ECHC.