缺氧诱导因子-1αmRNA和蛋白在妊高征胎盘组织中表达定位

Localization of hypoxia-inducible factor-1α protein and mRNA in preeclamptic placentas

目的 :探讨缺氧诱导因子 1α( HIF- 1α)在妊高征胎盘组织细胞中的定位。方法 :收取 1 0例妊高征孕妇的胎盘组织 ,应用免疫组织化学方法 ,观察 HIF- 1α蛋白在妊高征胎盘组织细胞中的定位表达 ;应用原位杂交的方法 ,观察 HIF- 1α m RNA在妊高征胎盘组织细胞中的定位表达。结果 :在妊高征胎盘中 ,HIF- 1α蛋白主要表达在合体滋养细胞、血管内皮细胞的胞核和胞浆中 ;HIF- 1 α m RNA主要表达在合体滋养细胞和血管内皮细胞的胞浆中。结论 :HIF- 1 α在妊高征胎盘组织中表达主要集中在胎盘的合体滋养细胞和血管内皮细胞。妊高征胎盘中合体滋养细胞和血管内皮细胞表达 HIF- 1 α。

Objective: To investigate the localization of hypoxia-inducible factor-1α(HIF-1α) protein and mRNA in preeclamptic placentas. Methods: Ten placental samples from preeclampsia were obtained. The location of HIF-1α protein in the preeclamptic placentas was observed by immunohistochemistry; the location of HIF-1α mRNA was observed by in situ hybridization. Results: The HIF-1α protein was mainly located in the nuclei and cytoplasm of syncytiotrophoblast and fetoplacental vascular endothelium in the preeclamptic placentas; but the HIF-1α mRNA was mainly located in the cytoplasm of syncytiotrophoblast and fetoplacental vascular endothelium in the preeclamptic placentas. Conclusions: The localization of HIF-1α was mainly in syncytiotrophoblast and fetoplacental vascular endothelium in the preeclamptic placentas. The localization of HIF-1α in syncytiotrophoblast and fetoplacental vascular endothelium of preeclamptic placentas may be related to pathogeny or pathology of preeclampsia.