用PCR方法检出蚤类携带巴尔通体

Molecular Evidence of Bartonella Species From Fleas in Yunnan

目的 调查我国巴尔通体宿主动物体表寄生蚤类是否携带巴尔通体。方法 2 0 0 3年 6～ 7月在云南省大理州云龙县居民区采集家猫、狗、鼠类等体表寄生蚤 ,用 3对巴尔通体属特异性引物BhCS .781p BhCS .113 7n、Bh .3 11p Bh .45 2n和TIle .45 5 p TAla .885n进行聚合酶链反应 (PCR) ,扩增巴尔通体gltA和16S～ 2 3SrRNAITS中部分核酸片段 ,检测采集的蚤类是否感染巴尔通体。结果 共采集 2 5 1只寄生蚤 ,包括猫栉首蚤、人蚤、缓慢细蚤等 7个常见蚤种 ,从 1组猫栉首蚤和 1组缓慢细蚤中扩增出目标带 ,证实有巴尔通体感染。结论 猫栉首蚤和缓慢细蚤能够感染巴尔通体 ,是该种病原体的潜在传播媒介 ,间接表明当地家猫和鼠类动物存在巴尔通体感染。

Objective Most known Bartonella species are arthropod borne and cat fleas can carry and transmit this organism from cats to cats. The study intended to make clear whether some Bartonella species, which are emerging pathogens, could be carried or transmitted by fleas in natural enviroments in China. Methods In this study, fleas were collected from domestic cats, dogs and rats hosts from June to July 2003 in Yunlong county, Yunnan province. PCR assay with genus Bartonella specific primers were applied to Bartonella detection in every group of fleas which were same species and came from one captured animal. Results Ctenocephalides felis, C.orientis, Pulex irritans,Xenopsylla cheopis, Leptopsylla segnis, Ctenophthalmus quadratus, Neopsylla specialis specialis collected from the host animals were 251. One group of Ct.felis including 8 tested fleas were PCR positive with three pairs of primers for Bartonella species. Five Leptopsylla segnis from a Rattus tanezumi flavipectus were also amplified DNA fragments of Bartonella species. Conclusion These findings indicate that fleas associated with cats and rats can carry Bartonella species and may play an important role in this pathogen transmission among animals and humans in China.