不同地区草原革蜱基因组DNA多态性研究

Studies on Polymorphic of Genomic DNA of Different Strains of Dermacentor nuttalli

目的 研究我国不同地区草原革蜱是否有种型差异。方法 从新疆阿尔泰、甘肃省武威、平凉地区采集草原革蜱标本 ,并分别提取它们的DNA ,选取 9条随机排列碱基序列片段 ,利用随机扩增多态DNA技术 (RandomAmplifiedPolymorphicDNA ,RAPD) ,确定聚合酶链反应 (PCR)总体积、反应条件。将扩增产物制备成DNA图谱 ,比较不同地区草原革蜱基因组DNA的多态性 ,从分子水平对不同地区草原革蜱进行差异分析。结果 3个地区的草原革蜱部分DNA条带相同 ,但也有完全不同的DNA条带。结论 3个地区的草原革蜱DNA存在差异。

Objective To study whether there are intraspecific differences among the strains of D.nuttalli from three different areas. Methods The specimens of D.nuttalli were collected from Aertai of Xinjiang autonomous region, Wuwei and Pingliang of Gansu province,and the genomic DAN was extracted respectively from them. The total volume of reaction and conditions of RAPD PCR were defined, 9 primers with different arbitrary single chain polynucleotide sequences were selected,the genomic DNA of three strains of D.nuttalli was respectively amplified by RAPD,DNA fingerprint maps were constructed with the amplified products, the polymorphism DNA of three strains of D.nuttalli was compared,the diversities of three trains of D.nuttalli were analyzed at DNA level. Results Some of the amplified DNA fragments are same among three strains,but the other DNA fragments are completely unique to every strain. Conclusion The diversities of genomic DNA among three strains of D.nuttalli exist.