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应用脂质体介导技术改变重组杆状病毒感染方法

An improved inoculation technique for inoculating recombinant baculovirus into silkworm, Bombyx mori, using lipofectin
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摘要 昆虫 重组杆状病毒表达系统是有效的真核表达系统之一 ,广泛应用于重组蛋白的生产。目前常采用将重组病毒直接注射入家蚕体内的方法进行感染表达 ,在实际操作过程中很容易造成病毒对环境的污染 ,具有潜在的危险性。为了严格控制作业环境重组病毒的扩散和潜在污染 ,开发安全、有效的感染方法显得非常必要。本研究直接将病毒基因组DNA导入家蚕体内取得同样的感染效果 ,探讨了利用阳离子脂质体介导下的避免病毒污染和提高感染效果的感染方法。 Insect-Baculovirus Expression System is one of the most effective eukaryotic expression systems, which has been widely used to produce a lot of recombinant proteins. The current inoculation method is to inject recombinant virus directly into silkworm larvae, resulting in possibly biological danger due to virus contamination to environment during the practical operation. Therefore, it is necessary to develop safe infection methods in order to control the diffusion of recombinant virus. In this experiment, we tried to introduce directly the genome DNA of baculovirus, instead of virus itself, into silkworm Bombyx mori larvae and got the same infection result. By using cationic lipofectin reagent, a new safe and effective infection technique without virus contamination was developed.
作者 吴小锋 曹翠平 崔为正
机构地区 浙江大学动物科学学院特种经济动物科学系 山东农业大学蚕学系
出处 《昆虫学报》 CAS CSCD 北大核心 2005年第1期143-146,共4页 Acta Entomologica Sinica
基金 国家留学回国人员科研启动基金 农业部"948"项目 (2 0 0 3 Z5 0 ) 浙江省科技厅重点国际合作项目 (2 0 0 2C2 40 0 3 )
关键词 重组杆状病毒 家蚕 感染技术 脂质体 Recombinant baculoviruses silkworm inoculation technique lipofectin
分类号 S881.2 [农业科学—特种经济动物饲养]
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参考文献10

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二级参考文献16

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昆虫学报
2005年 第1期

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