血小板源性生长因子对新生大鼠高氧肺损伤的影响

Effect of Platelet-Derived Growth Factor on Lung Injury in Hyperoxia-Exposed Ne wborn Rats

目的 探讨血小板源性生长因子 (PDGF)在新生大鼠高氧肺损伤中时空作用机制。方法 应用免疫组织化学法定位PDGF表达 ,反转录 聚合酶链反应 (RT PCR)检测各时间点 (生后 4、7、10、14d)PDGFmRNA水平。结果 随日龄增加 ,PDGF A、 B含量发生不同的变化。高氧暴露下PDGF BmRNA含量及其表达均显著高于空气组 (P <0 .0 5 ,<0 .0 1)。而PDGF AmRNA水平及其表达却显著低于空气组 (P <0 .0 5 ,P <0 .0 1)。结论 高氧降低PDGF A水平 ,增加PDGF B含量。PDGF A、 B共同参与高氧暴露下肺组织损伤过程 ,是肺发育阻滞的重要因素。

Objective To investigate temporal and spatial effective mechanism of platelet-derived grow th factor(PDGF) to lung injury in newborn rats with hyperoxia-exposed. Methods Immunohistochemical methods were used to locate expression of PDGF,levels of PDG F mRNA were measured by reverse transcription polymerase chain reaction(RT-PCR) at age 4,7,10,14days. Results With the increasing ages,levels of PDGF-A and PDGF-B changed differently.Compa red with air group,levels of PDGF-B and PDGF-B mRNA increased significantly(P <0.05,P<0.01),but PDGF-A and PDGF-A mRNA reduced markedly (P<0.05,P <0.01)in hyperoxia-exposed group.Conclusions Levels of PDGF-A reduce and that of PDGF-B increase under hyperoxia exposure,P DGF-A、-B are involved in lung injury process and the importment factors in th e inhibition of lung development. J Appl Clin Pediatr,2005,20(2):113-115