摘要
目的 探讨Bid基因在新生大鼠缺氧缺血性脑损伤 (HIBD)中的动态变化。方法 通过建立新生大鼠HIBD动物模型 ,采用RT PCR技术检测缺氧缺血后不同时间点缺血侧脑组织中Bid基因表达的变化。结果 正常组中无Bid基因表达 ,缺氧缺血组Bid基因表达明显上调 ,且随着缺氧缺血后时间的延长 ,Bid基因表达呈动态变化缺氧缺血后 6h其表达开始增加 ,2 4h达高峰 ,48～ 72h有所回落。结论 脑缺氧缺血引起的神经细胞凋亡可能与Bid基因表达上调有关。
Objective To study the changes and significance of Bid gene expression in hypoxic-ischemic brain damage (HIBD) in neonatal rats.Methods The animal models of HIBD were made. Total RNA from ipsilateral cerebral hemisph ere was extracted.RT-PCR was used to evaluate the levels of Bid gene expression at different time points after hypoxic-ischemic. Results The levels of Bid mRNA were higher in HIBD rats than those in normal rats.The ex pression of Bid mRNA in ipsilateral hemiphere following cerebral hypoxic-ischem ic began to increase at 6 h, increased to the highest level at 24 h, and then gr adually returned.Conclusion The upregulation of Bid mRNA can play a role in the induction of apoptosis fol lowing cerebral hypoxic-ischemic. J Appl Clin Pediatr,2005,20(2):121-122
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第2期121-122,共2页
Journal of Applied Clinical Pediatrics
基金
江苏省教育厅自然科学基金资助 (98KJD3 2 0 0 0 6)
关键词
脑缺氧
脑缺血
Bid基因
细胞凋亡
cerebral hypoxia
cerebral ischemia
Bid gene
apoptosis
