聚合酶链反应在检测脑性瘫痪患儿人巨细胞病毒感染中的应用

Application of Polymerase Chain Reaction in Checking Human Cytomegalovirus o f Cerebral Palsy Children

目的 探讨聚合酶链反应 (PCR)在检测脑性瘫痪 (CP)患儿人巨细胞病毒 (HCMV)感染中的意义。方法 对 5 6例CP患儿用PCR检测尿巨细胞病毒 (CMV)DNA ,病毒分离检测尿标本 ,观察CMV特征性致细胞病变效应 (CPE) ,ELISA检测血清CMVIgM和IgG。结果 PCR检测CMVDNA阳性率为 5 3 .6% ,9例CMV病毒分离结果阳性 ;CMVIgM、IgG阳性率分别为 12 .5 %、3 7.5 % ;病毒分离 :CMVIgM阳性与CMVDNA阳性的符合率分别为 10 0 %、10 % ,前两者与后者比较均有显著差异 (P <0 .0 1) ;CMVIgG阳性与PCR阳性符合率为 6.7% ,两者无显著差异 (P >0 .0 5 )。结论 PCR检测CMVDNA能够早期快速检测到CP患儿CMV感染 ,可应用于CP患儿中CMV感染的检测 ,为早期干预提供可靠依据。

Objective To explore the clinical application and significance of polymerase chain reactio n(PCR) in checking human cytomegalovirus (HCMV)in cerebral palsy children. Methods Collecting urine and serum of 56 cerebral palsy (CP) children,using PCR to detec t CMV DNA from urine,isolate CMV from urine,and indirect enzyme-linked immuno so rbent assay(ELISA) detecting CMV IgM、IgG of serum.Results In 56 cases,53.6%cases were CMV DNA positive,there were 9 cases CMV isolation,o bserving CMV characteristic cytopathic effect (CPE) and the positive value of se rum CMV IgM、IgG was 12.5%,37.5% respectively.The positive value in isolation o f the virus and CMV IgM was 100%,10% corresponding with that of CMV DNA.Comp ared the 2 former with the latter,it was significant(P<0.01);the positiv e value of CMV IgG was 6.7%corresponding with that of CMV DNA and the result w as no significance(P>0.05).Conclusions Using PCR can detect CMV DNA from CP children with CMV infection quickly.It can apply in detecting CMV in CP and provide credible evidence for intervention as f ar as early in children with CP. J Appl Clin Pediatr,2005,20(2):157-159