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不同成熟期大鼠对戊四氮反复致性损伤的耐受性研究 被引量:1

Study on Resistance of Brains at Different Developmental Stages Following Penty lenetetrazol-Induced Recurrent Seizures in Rats
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摘要 目的 观察不同成熟期大鼠反复惊厥后海马结构的变化 ,并研究发育鼠对惊厥性损伤的耐受性。方法 对生后 10、60d(P10、P60 )实验组大鼠用戊四氮反复点燃 5d ,设P10、P60生理盐水对照组 ;观察两实验组惊厥潜伏期、潜伏发作期、惊厥持续时间 ;海马神经元形态学改变 :海马CA1 、CA3、DG及门区进行细胞计数 ;组织化学染色观察苔藓纤维发芽 ,分别进行实验组间、实验组与对应对照组间比较。结果  1.P10惊厥发作潜伏期 (1.0 7± 0 .5 5 )min、潜伏发作期 (14 .0 0± 2 .70 )min较P60 [(8.2 7± 1.48)、(4 .16± 5 .2 2 )min]短 ,惊厥持续时间 (4 6.3 3± 7.65 )min较成年鼠 (17.0 7± 5 .66)min明显延长 ;2 .P10实验组与对照组比较海马齿状回CA1 、CA3区无明显神经元丢失 ,齿状回DG区颗粒细胞数实验组 (2 3 .2 5± 3 .0 6)较对照组 (16.2 5± 1.5 8)增多。而P60实验组CA1 、CA3区神经元计数 (8.2 2± 1.88、5 .62±l.68)较对照组 (6.3 1± 1.5 0、3 .62± 1.40 )明显减少 ,DG区无明显改变 :3 .Timm染色CA3区锥体层苔藓纤维发芽两实验组均有增加 ,但P60 (3 .2 5±l.0 3 )较P10 (l.5± 0 .92 )更明显 ;以上指标经比较均有显著差异(P均 <0 .0 5 )。结论  1.虽幼鼠较易发生惊厥 ,但致后海马神经元损伤。 Objective To determine if there is an early developmental resistance to seizure-induced hi ppocampal damage. Methods Five daily pentylenetetrazol-indu ced convulsions in immature rats beginning at postnatal day P10,P60 groups.In b oth groups, the latency of seizure, the latency of Ⅳ/Ⅴ grade, the lasting time of seizure and mortality of rats after seizure were used to measure sensitivity of seizure or the resistance to brain damage. Conventional histopathological me thod was utilized to observe morpbological changes and cell counting of dentate granule cells, CA 3,CA 1 and hilar neurnns. Timm histochemical technique was a dopted to study mossy fiber sprou- ting.Results 1.In the both groups(P10,P60),there were significant differences in the latency of seizure (1.07±0.55 vs 8.27±1.48 P<0.05 ),the latency of Ⅳ/Ⅴ grad e (14±2.70 vs 41.6±5.22 P<0.05),the lasting time of seizure(46.33±7. 65 vs 17.07±5.66 P<0.05).2.In immature rats(10 days old),cell counting of CA 1, CA 3 and hilar neurons demonstrated no differences from controIs in rats,whereas adult rats with daily seizures had a significant decrease in CA 1, CA 3 neurons (8.22±l.88、5.62±1.68 vs 6.31± 1.50、3.62±1.40).In adul t rats,cell counting of dentate gradule cells demonstrated no differences from c ontrols in ratas, whereas immiture rats with daily seizures had a significant in crease(23.25±3.058 vs 16.25±1.58).3.Based on Timm staining,prominent sprou ting was seen in the CA 3 stratum pyramidal layer in all experimental rats havi ng 5 daily seizures, regardless of the age. But the degree of sprouting had sign ificant differences between the experimental groups (3.25±1.03 vs 1.5±0.92 P<0.05).Conclusions 1.Though immature rats have prolonged seizures and increased susceptibility th an adult rats, resistance of hippocanipus neuron loss and mossy fiber sprouting aiterations inimmature rats is higher than adult rats. The higher resistance is based on hippocanipus morphologic alterations of immature rats followting rec urrent seizures.2.In the immature rats,recurrent seizures can also result in de ntate granule cell neurogenesis without loss of neurons,which can play an effect on mechanism of mossy fiber sprouting in immature rats. J Appl Clin Pediatr,2005,20(2):160-162
出处 《实用儿科临床杂志》 CAS CSCD 北大核心 2005年第2期160-162,i004,共4页 Journal of Applied Clinical Pediatrics
基金 湖北省自然科学基金资助项目 (2 0 0 4ABA2 3 4)
关键词 海马 惊厥 大鼠 戊四氮 hippocampus seizure rats pentylenetetrazol
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参考文献7

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同被引文献12

  • 1黄亚玲,孙丹,刘亚黎.戊四氮反复点燃致不同发育期大鼠海马损伤的病理改变[J].中华儿科杂志,2005,43(12):930-934. 被引量:5
  • 2陈静,袁宝强.戊四氮诱导发育鼠癫癎持续状态后海马神经发生及MK-801的影响[J].中国当代儿科杂志,2006,8(5):421-424. 被引量:6
  • 3李国强,刘仕勇,周政,陈军花.慢性颞叶癫痫模型鼠海马结构形态学的变化[J].第三军医大学学报,2007,29(2):125-128. 被引量:7
  • 4Shapiro LA, Ribak CE. Newly born dentate granule neurons after pilocarpine- induced epilepsy have hilar basal dendrites with immature synapses[J]. Epilepsy Res,2006,69( 1 ) : 53 -66.
  • 5Pierce JP,Mehon J,Punsoni M,et al. Mossy fibers are the primary sourece of afferent input to ectopie granule cells that are born after pilocarpine - induced seizures [ J ]. Exp Neurol,2005,196 ( 2 ) : 316 - 331.
  • 6Hussenet F, Boyet S, Nehlig A. Long - term metabolic effects of pentylenetetrazol -induced status epilepfieus in the immature rat [ J ].J Neurosci, 1995, 67(2) : 455 -461.
  • 7Fathollahi Y, Motamedi F, Semnanian S, et al. Examination of persistent effects of repeated administration of pentylenetetrazolon on rat hippecampal CA1 : Evidence from in vitro study on hippocampal slices[J]. Brain Res, 1997, 758(1 -2) : 92 -98.
  • 8Cilio MR, Sogawa Y, Cha BH, et al. Long - terra effects of status epilepticus in the immature brain are specific for age and model[ J]. Epilepsia, 2003,44(4) : 518 -528.
  • 9Kang TC, Kim DS, Kwak SE,et al.Epileptogenic roles of astroglial death and regeneration in the dentate gyms of experimental temporal loke epilepsy[J]. Glia, 2006, 54(4) : 258 -271.
  • 10Ben - Ari Y, Holmes GL. Effects of seizures on developmental processes in the immature brain [ J ]. Lancet Neurol, 2006, 5 ( 12 ) : 1055 -1063.

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