摘要
采用鸡胚成纤维细胞(CEF)培养增殖首次从湖北省云梦县分离的鹦鹉幼雏病病毒(Budgerigar fledgling disease virus,BFDV)分离株(BFDV-HBYM02),经PCR分段扩增法获得全基因组并完成序列测定.HBYM02株全序列测定结果与GenBank中仅有的六株BFDV全序列进行同源性与进化分析.经BLAST分析,HBYM02株与其他六株BFDV同源性为98%～99%,为同一个基因型.运用Phylip3.5软件构建进化树,分析显示,来源于不同宿主的BFDV与宿主关系紧密,与地理分布没有明显的相关性.
The genome of BFDV strain HBYM02 isolated from Hubei province was sequenced and analyzed. The sequence suggested BFDV isolated from different hosts belonged to one genotype. A phylogenetic tree based on the genomic nucleotide sequences of seven isolates was constructed. This tree showed two main branches. Taking into account of the relation of the hosts, the tree suggested BFDV formed a host-specific evolution, and it was impossible to delineate a geographically based evolution.
出处
《中国病毒学》
CSCD
2005年第1期46-49,共4页
Virologica Sinica
基金
中国科学院知识创新项目资助 (KSCX2 SW 302 3)
湖北省科技攻关重点基金项目资助 (2001AA201B01)
关键词
鹦鹉幼雏病病毒
序列分析
进化
Budgerigar fledgling disease virus(BFDV)
Sequence analysis
Evolution
