摘要
用AFLP方法对灰背栎 (Quercussenescens) 8个居群进行了遗传多样性、居群遗传结构研究。TFPGA软件分析两组引物组合共产生 12 5个位点 ,其中 94个为多态位点 ,多态位点百分率为 75 2 % ,发现灰背栎居群的遗传变异水平有随着海拔升高而遗传多样性下降的趋势。Arliquin 2 0 0 0中的AMOVA分析表明灰背栎居群间分化大 ,分化指数达 φst=0 2 95 6。
The population variation and structure of 115 plants representing 8 populations of Quercus senescens Hand.-Mazz. were investigated by amplification fragment length polymorphism (AFLP) analysis.Using TFPGA software,94 polymorphic phenotypic patterns (75.2%) was obtained using 2 AFLP primer combinations and it was found that genetic variation of different population was negatively correlated with altitudes.Analysis of the genetic structure of populations carried out using analysis of molecular variation (by Arliquin 2.000 software),It showed that there was a high significant population differentiation (φ_(st)=0.2956).And neighbour-joining dendragram also be constructed based on 2 primer combinations by PAUP software.
出处
《云南植物研究》
CSCD
北大核心
2005年第1期49-58,共10页
Acta Botanica Yunnanica
基金
国家自然科学基金项目 (30 170 0 77
4 0 332 0 2 1)
国家重点基础研究发展计划 (973)项目 (2 0 0 3CB4 15 10 2 )资助
关键词
灰背栎
AFLP
遗传多样性
遗传结构
纵向岭谷区
Quercus senescens
Amplified fragment length polymorphism(AFLP)
Genetic variation
Genetic structure
Longitudinal Range-Gorge
